02091nas a2200229   4500008004100000245013800041210006900179260001200248300001400260490000800274520130400282653000901586653002201595653002201617653002301639653002801662100001601690700001301706700001101719700001401730856011701744       2010                        eng d00aGenetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila Hand.-Mazz. revealed by AFLP markers0 aGenetic diversity and population structure of the endangered alp  c2010///  a127 - 1390 v2903 aIsoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered quillwort endemic to the Qinghai-Tibetan Plateau. Genetic variation and population structure of 12 I. hypsophila populations were examined by using amplified fragment length polymorphism (AFLP) markers. Eight primer pairs produced a total of 472 unambiguous bands, of which 229 (48.5%) were polymorphic. Intra-population genetic variation of Isoetes hypsophila was low (PP = 14.9%, HE = 0.039, hs = 0.084 and I = 0.061). The F statistics calculated by different approaches consistently revealed high genetic differentiation among populations, contributing approximately 50% of total gene diversity. Four genetic groups corresponding to four geographic regions were detected, indicating significant geographic structure. Our results suggest that both ongoing evolutionary forces (e.g., inbreeding system, bottlenecks resulting from fluctuation of water level) and historical events (e.g., orogenic movements which reduce gene flow among populations, repeated population retreat, and recolonization during ice ages) may have contributed to the genetic structure of I. hypsophila. In conservation, attention should be paid to preserving every population, and intra-region translocations can currently be recommended. © 2010 Springer-Verlag.10aAFLP10aAquatic quillwort10aGenetic structure10aIsoetes hypsophila10aQinghai-Tibetan Plateau1 aChen, Y.-Y.1 aLiao, L.1 aLi, W.1 aLi, Z.-Z.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-78149498133&partnerID=40&md5=4c18cfb671d1d50e919e742e3bf8659302034nas a2200253   4500008004100000245013900041210006900180260001200249300001200261490000700273520118700280653001701467653001401484653002201498653002301520653001401543653003301557100001401590700001401604700001501618700001401633700001601647856011701663       2013                        eng d00aGenetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila (Isoetaceae) revealed by SSR analysis0 aGenetic diversity and population structure of the endangered alp  c2013///  a11 - 200 v473 aIsoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered alpine quillwort endemic to the Qinghai-Tibetan Plateau (QTP). A total of 247 individuals of I. hypsophila from 11 populations were analyzed using ten pairs simple sequence repeats (SSR) markers. Seventy-seven alleles were recorded, with an average of 7.7 alleles per locus (ranging from 3 to 12) and the expected heterozygosity (HE) ranged from 0.392 to 0.536 (average 0.471). Clear population structure among I. hypsophila populations was found and the proportion of genetic differentiation among populations accounted for about 20% of total genetic diversity (FST = 0.178; probability of allelic co-ancestory Fcoal = 0.238). This genetic structure was probably due to limited gene flow hindered by complex terrains, bottlenecks resulting from water fluctuation in habitats, and repeated population retreat and recolonization during the ice ages. In conservation, four management units corresponding to four geographic regions were suggested, and the populations from different management units with higher genetic variation should be the priority targets of both in situ and ex situ conservation. © 2012 Elsevier Ltd.10aConservation10aGene flow10aGenetic structure10aIsoetes hypsophila10aQuillwort10aSimple sequence repeat (SSR)1 aLi, X.-L.1 aLi, S.-C.1 aChu, H.-J.1 aLi, Z.-Z.1 aChen, Y.-Y.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84875089755&partnerID=40&md5=c1cc33debe532b0ac82fdd9916012f95