02139nas a2200145   4500008004100000245010100041210006900142260001200211300001400223490000700237520160000244100001701844700001601861856011601877       2000                        eng d00aIsozymes of the Isoetes riparia complex, I. Genetic variation and relatedness of diploid species0 aIsozymes of the Isoetes riparia complex I Genetic variation and   c2000///  a235 - 2590 v253 aIsozymes were examined in the six aquatic (including amphibious) diploid species of Isoetes of eastern North America in order to quantify their levels and patterns of genetic variation, to determine their genetic distinctness, and to evaluate the utility of allozymes in addressing ancestry in the I. riparia complex. Twenty-six populations representing diploid species, I. echinospora, I. engelmannii, I. flaccida, I. mattaponi, I. prototypus, and I. valida were compared for eleven enzymes coded by 16 interpretable loci. Levels of genetic variation in populations tended to be low ranging from none (means: P = 0.0, A = 1.0, H = 0.0) in I. echinospora to moderate (means: P = 22.9, A = 1.4, H = 0.093) in I. flaccida. Genetic variation in polymorphic species tended to be distributed more among than within populations, as indicated by high F(st) values (F(st) > 0.607 in all polymorphic species except I. mattaponi, for which F(st) = 0.272). The genetic identities of conspecific populations were high, with mean values ranging from I = 0.779/S = 0.733 in I. flaccida to I = 1.000/S = 1.000 in I. echinospora. Interspecific genetic identities were lower, with means ranging from I = 0.438/S = 0.429 between I. mattaponi and I. prototypus to I = 0.736 between I. valida and I. flaccida, and S = 0.699 between I. valida and I. prototypus. Thus the six diploids were found to be genetically distinct, corroborating their recognition based on morphology. Evidence from this study indicates that isozymes may be informative in diagnosing ancestry of allopolyploids in the Isoetes riparia complex.1 aCaplen, C.A.1 aWerth, C.R.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0034042682&partnerID=40&md5=ed389e88047f4d191722332cdcce26a9