02016nas a2200241   4500008004100000245012000041210007100161260001200232300001400244490000700258520124700265653000901512653002001521653001601541653001201557653002401569653001001593100001201603700001301615700001301628700001601641856011701657       2009                        eng d00aSystematic evaluation of isoëtes asiatica makino (isoëtaceae) based on AFLP, nrITS, and chloroplast DNA sequences0 aSystematic evaluation of isoëtes asiatica makino isoëtaceae base  c2009///  a501 - 5100 v523 aOn the basis of amplified fragment length polymorphism (AFLP) and nucleotide sequence data from nuclear ribosomal internal transcribed spacer (nrITS) and three chloroplast DNA regions (rbcL, cpITS, and trnS-psbC spacer), we investigated the species delimitation and the evolutionary lineage of Isoëtes asiatica from Hokkaido, Japan. The neighbor-joining (NJ) dendrogram based on AFLP markers revealed the well-defined clusters (bootstrap value = 100%) of I. asiatica. Results from the principal component analysis are largely congruent with those obtained in the NJ dendrogram. The maximum parsimony analysis, based on data from nrITS and three chloroplast DNA sequences, supported a monophyly of three species, I. asiatica, Isoëtes echinospora, and Isoëtes maritima from Hokkaido, Kamchatka, and Alaska regions. The distinct species status of I. asiatica was also well supported in the combined chloroplast DNA phylogeny. Therefore, I. asiatica appear to represent example of gradual speciation due to spatial isolation of ancestral populations followed by genetic divergence. Our results also suggest that I. asiatica is probably not the ancestral diploid of the polyploids occurring in East Asia. © The Botanical Society of Korea 2009.10aAFLP10aChloroplast DNA10aI. asiatica10aIsoetes10aMolecular phylogeny10aNrITS1 aKim, C.1 aNa, H.R.1 aShin, H.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-71249137227&partnerID=40&md5=029dcd01eab0eacc2d8c97b3953cb1ba