@article {47,
	title = {High allozyme diversity and unidirectional linear migration patterns within a population of tetraploid Isoetes sinensis, a rare and endangered pteridophyte},
	journal = {Aquatic Botany},
	volume = {90},
	year = {2009},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {52 - 58},
	abstract = {Isoetes sinensis (Isoetaceae), an aquatic quillwort which occurs only in two fragmented sites of China as an allotetraploid, is critically endangered. Genetic variation among eight subpopulations of I. sinensis was examined in the Xin{\textquoteright}an River (119{\textdegree}14'-15'E, 29{\textdegree}28'N) by using allozyme polymorphism. Eighteen loci of 10 enzyme systems were examined and used for the analysis of population genetic parameters. As expected for allotetraploids, fixed heterozygosity was found at four loci. A high level of genetic diversity was observed in the population, with mean number of alleles per loci of 1.8, and mean percentage of polymorphic loci of 55.6\%, which were much higher than the average values in fern species. The genetic variation within each subpopulation was not positively correlated with its size, which may be explained by high gene flow (Nm = 2.57), clonal reproduction and fixed heterozygosity of allopolyploid. The I. sinensis population contained high clonal diversity (PD = 0.39, D = 0.95), indicating the successful seedling recruitment of the population. Significant positive relationship was detected between clonal diversity and the size of subpopulation. Partitioning the genetic diversity indicated that 91.1\% of the genetic variation was within subpopulations and only 8.9\% existed among subpopulations. The migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by a source-sink model, but with unidirectional gene flow among subpopulations underlined by hydrochoric force. The results were then discussed in relation to both in situ and ex situ conservation efforts of the population. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Allotetraploid, Genetic diversity, Hydrochory, Isoetes, Riparian plant, Unidirectional migration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-54249105798\&partnerID=40\&md5=962f14606c468408a8b8d98333cd3292},
	author = {Chen, Y.-Y. and Yang, W. and Li, W. and Li, Z.-Z. and Huang, H.-W.}
}
@article {30,
	title = {Genetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila Hand.-Mazz. revealed by AFLP markers},
	journal = {Plant Systematics and Evolution},
	volume = {290},
	year = {2010},
	note = {Cited By (since 1996):6Export Date: 11 November 2013Source: Scopus},
	month = {2010///},
	pages = {127 - 139},
	abstract = {Isoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered quillwort endemic to the Qinghai-Tibetan Plateau. Genetic variation and population structure of 12 I. hypsophila populations were examined by using amplified fragment length polymorphism (AFLP) markers. Eight primer pairs produced a total of 472 unambiguous bands, of which 229 (48.5\%) were polymorphic. Intra-population genetic variation of Isoetes hypsophila was low (PP = 14.9\%, HE = 0.039, hs = 0.084 and I = 0.061). The F statistics calculated by different approaches consistently revealed high genetic differentiation among populations, contributing approximately 50\% of total gene diversity. Four genetic groups corresponding to four geographic regions were detected, indicating significant geographic structure. Our results suggest that both ongoing evolutionary forces (e.g., inbreeding system, bottlenecks resulting from fluctuation of water level) and historical events (e.g., orogenic movements which reduce gene flow among populations, repeated population retreat, and recolonization during ice ages) may have contributed to the genetic structure of I. hypsophila. In conservation, attention should be paid to preserving every population, and intra-region translocations can currently be recommended. {\textcopyright} 2010 Springer-Verlag.},
	keywords = {AFLP, Aquatic quillwort, Genetic structure, Isoetes hypsophila, Qinghai-Tibetan Plateau},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78149498133\&partnerID=40\&md5=4c18cfb671d1d50e919e742e3bf86593},
	author = {Chen, Y.-Y. and Liao, L. and Li, W. and Li, Z.-Z.}
}
@article {21,
	title = {Microsatellite analysis reveals the genetic structure and gene flow of the aquatic quillwort Isoetes sinensis, a critically endangered species in China},
	journal = {Aquatic Botany},
	volume = {96},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {52 - 57},
	abstract = {Isoetes sinensis is a critically endangered aquatic quillwort, occurring in two fragmented sites of China (Xiuning county of Anhui; Jiande county of Zhejiang). During a five-year period (2004-2009), the areas and sizes of the two populations diminished dramatically due to intensive human activities. Genetic structure of the species was investigated using simple sequence repeat makers (SSRs). For seven nuclear microsatellites, high levels of genetic diversity were found within populations (H E=0.324-0.447). Strong genetic differentiation was detected between populations (G ST=0.376), while weak genetic differentiation was found within populations (G ST=0.026-0.080). Notably, in contrast to the source-sink model suggested by previous study (Chen et al., 2009), the migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by the linear symmetrical non-adjacent flow model (LSNF), which indicates that intensive human activities of recent years have greatly affected the gene exchange pattern among I. sinensis subpopulations. {\textcopyright} 2011 Elsevier B.V.},
	keywords = {Allotetraploid, Genetic diversity, Isoetes, Migration pattern, Riparian plant, Stepping-stone model},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84855293635\&partnerID=40\&md5=824e647a2989ecda4486a69d98cdaac2},
	author = {Chen, Y.-Y. and Kong, D.-R. and Huang, C.-H. and Xu, Y.-X. and Li, Z.-Z.}
}
@article {8,
	title = {Genetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila (Isoetaceae) revealed by SSR analysis},
	journal = {Biochemical Systematics and Ecology},
	volume = {47},
	year = {2013},
	note = {Export Date: 11 November 2013Source: Scopus},
	month = {2013///},
	pages = {11 - 20},
	abstract = {Isoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered alpine quillwort endemic to the Qinghai-Tibetan Plateau (QTP). A total of 247 individuals of I. hypsophila from 11 populations were analyzed using ten pairs simple sequence repeats (SSR) markers. Seventy-seven alleles were recorded, with an average of 7.7 alleles per locus (ranging from 3 to 12) and the expected heterozygosity (HE) ranged from 0.392 to 0.536 (average 0.471). Clear population structure among I. hypsophila populations was found and the proportion of genetic differentiation among populations accounted for about 20\% of total genetic diversity (FST = 0.178; probability of allelic co-ancestory Fcoal = 0.238). This genetic structure was probably due to limited gene flow hindered by complex terrains, bottlenecks resulting from water fluctuation in habitats, and repeated population retreat and recolonization during the ice ages. In conservation, four management units corresponding to four geographic regions were suggested, and the populations from different management units with higher genetic variation should be the priority targets of both in situ and ex situ conservation. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Conservation, Gene flow, Genetic structure, Isoetes hypsophila, Quillwort, Simple sequence repeat (SSR)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84875089755\&partnerID=40\&md5=c1cc33debe532b0ac82fdd9916012f95},
	author = {Li, X.-L. and Li, S.-C. and Chu, H.-J. and Li, Z.-Z. and Chen, Y.-Y.}
}
