@article {65,
	title = {Ecology and conservation of the endangered quillwort Isoetes sinensis in China},
	journal = {Journal of Natural History},
	volume = {39},
	year = {2006},
	note = {Cited By (since 1996):2Export Date: 11 November 2013Source: Scopus},
	month = {2006///},
	pages = {4069 - 4079},
	abstract = {Isoetes sinensis Palmer is an East Asia endemic fern that faces impending extirpation from the expansive area of Mainland China. Presently, only two natural populations are known to exist in this vast region. Both populations have decreased by about 50\% within the last 4 years. This reduction is most probably related to the decline in the size of the wetlands since reproductive activity, spore production, and recruitment were noticeably lower at the microsites occurring in smaller ponds. The decline in population size seems to be associated with succession from marsh to dry land at the habitats of both populations. The study revealed that individuals in both populations produced copious numbers of spores suggesting adequate fecundity, furthermore high levels of spore germination were obtained in laboratory experiments. However, inefficient spore dispersal appears to have contributed to the limited spread of the species. The sex ratio in both populations was male-biased. Several factors are identified here as posing a threat to Isoetes sinensis populations in China, chiefly the loss of habitat due to infrastructure construction and competition with adventive weed species for establishment on available sites. Disturbance by introduced mammals during browsing and trampling by livestock farm workers, as well as tourists, further contribute to the diminishing numbers of Isoetes sinensis at the study sites. {\textcopyright} 2005 Taylor \& Francis.},
	keywords = {China, Conservation, Ecology, Isoetes sinensis, Rare fern},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33646483501\&partnerID=40\&md5=91677e55ca7f81da659db6f0f6afe260},
	author = {Wang, J.-Y. and Gituru, R.W. and Wang, Q.-F.}
}
@article {67,
	title = {Genetic consequence of restricted habitat and population decline in endangered Isoetes sinensis (Isoetaceae)},
	journal = {Annals of Botany},
	volume = {96},
	year = {2005},
	note = {Cited By (since 1996):19Export Date: 11 November 2013Source: Scopus},
	month = {2005///},
	pages = {1265 - 1274},
	abstract = {{\textbullet} Background and Aims: Isoetes sinensis (Isoeteaceae) is a critically endangered aquatic quillwort in eastern China. Rapid decline of extant population size and local population extinction have occurred in recent years and have raised great concerns among conservationists. {\textbullet} Methods: Amplified fragment length polymorphisms (AFLPs) were used to investigate the genetic variation and population structure of seven extant populations of the species. {\textbullet} Key Results: Eight primer combinations produced a total of 343 unambiguous bands of which 210 (61{\textperiodcentered}2 \%) were polymorphic. Isoetes sinensis exhibited a high level of intra-population genetic diversity (H E) = 0{\textperiodcentered}118; hs = 0{\textperiodcentered}147; I = 0{\textperiodcentered}192; P = 35{\textperiodcentered}2 \%). The genetic variation within each of the populations was not positively correlated with their size, suggesting recent population decline, which is well in accordance with field data of demographic surveys. Moreover, a high degree of genetic differentiation (FST = 0{\textperiodcentered}535; G ST = 0{\textperiodcentered}608; θB = 0{\textperiodcentered}607) was detected among populations and no correlation was found between geographical and genetic distance, suggesting that populations were in disequilibrium of migration-drift. Genetic drift played a more important role than gene flow in the current population genetic structure of I. sinensis because migration of I. sinensis is predominantly water-mediated and habitat range was highly influenced by environment changes. {\textbullet} Conclusions: Genetic information obtained in the present study provides useful baseline data for formulating conservation strategies. Conservation management, including both reinforcement for in situ populations and ex situ conservation programmes should be carefully designed to avoid the potential risk of outbreeding depression by admixture of individuals from different regions. However, translocation within the same regional population should be considered as a measure of genetic enhancement to rehabilitate local populations. An ex situ conservation strategy for conserving all extant populations to maximize genomic representation of the species is also recommended. {\textcopyright} The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.},
	keywords = {AFLP, Genetic differentiation, Genetic diversity, Isoetes sinensis, Population structure, Pteridophyte},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27944462292\&partnerID=40\&md5=26404edf2320e666820f93f6dd369ebf},
	author = {Kang, M. and Ye, Q. and Huang, H.}
}
@article {53,
	title = {Irregular meiotic behavior in Isoetes sinensis (Isoetaceae), a rare and endangered fern in China},
	journal = {Caryologia},
	volume = {60},
	year = {2007},
	note = {Cited By (since 1996):3Export Date: 11 November 2013Source: Scopus},
	month = {2007///},
	pages = {358 - 363},
	abstract = {Chromosomal abnormalities including cytomixis occurred in meiotic process of pollen mother cells of many angiosperm species at high frequency, but similar phenomenon was rarely reported in low vascular plants. This study we investigated the microsporogenesis of Isoetes sinensis Palmer, a quillwort of ancient heterosporous lycopsids Isoetaceae plants. Irregular chromosomal behavior especially cytomixis was emphasized. It appeared in each stage of meiotic division from prophase I to telophase II. The cytoplasmic channels between microspore mother cells were narrow and stretched. The genetic material connection was maintained mainly by visible chromatin fiber. The cytomixis was just occurred between two adjacent microspore mother cells of same stages. A variety of other abnormal chromosomal behaviors including chromosomal stickiness, fragmentation, bridge, laggards, micronucleus, were observed, albeit with low frequency. All sorts of chromosomal abnormalities were counted at two stages, anaphase I and anaphase II. They were approximately 24.4\% and 26.9\% respectively. This number was slightly higher than that of microspore abortion which was about 14.8\%. I. sinensis was listed as a critical endangered species due to habitat fragmentalization, water pollution and wetland degradation. Human being activities are without doubt the immediate forces resulting in the population decreasing of I. sinensis. Whether the individual numbers dropping or habitat withdraw has consequently caused the producing of the overall high abnormalities of chromosomal behaviours is not determined yet. However, cytomixis and other irregular chromosomal phenomenon seemed not to be a natural process.},
	keywords = {Chromosomal abnormality, Cytomixis, Eastern China, Isoetes sinensis, Microsporogenesis},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-49449109278\&partnerID=40\&md5=10a888a1588bfcc9524b7cca21020912},
	author = {Heng-Chang, W. and Li, J.-Q. and He, Z.-C.}
}
@article {16,
	title = {Microsatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis},
	journal = {American Journal of Botany},
	volume = {99},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {e184 - e186},
	abstract = {Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di-and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. {\textcopyright} 2012 Botanical Society of America.},
	keywords = {Cross-amplification, Genetic diversity, Isoetaceae, Isoetes hypsophila, Isoetes sinensis, Microsatellite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84860573611\&partnerID=40\&md5=4de1802063e8ced0a9e32ab46966d215},
	author = {Li, Z. and Han, Q. and Chen, Y. and Li, W.}
}
