@article {38,
	title = {Systematic evaluation of iso{\"e}tes asiatica makino (iso{\"e}taceae) based on AFLP, nrITS, and chloroplast DNA sequences},
	journal = {Journal of Plant Biology},
	volume = {52},
	year = {2009},
	note = {Cited By (since 1996):2Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {501 - 510},
	abstract = {On the basis of amplified fragment length polymorphism (AFLP) and nucleotide sequence data from nuclear ribosomal internal transcribed spacer (nrITS) and three chloroplast DNA regions (rbcL, cpITS, and trnS-psbC spacer), we investigated the species delimitation and the evolutionary lineage of Iso{\"e}tes asiatica from Hokkaido, Japan. The neighbor-joining (NJ) dendrogram based on AFLP markers revealed the well-defined clusters (bootstrap value = 100\%) of I. asiatica. Results from the principal component analysis are largely congruent with those obtained in the NJ dendrogram. The maximum parsimony analysis, based on data from nrITS and three chloroplast DNA sequences, supported a monophyly of three species, I. asiatica, Iso{\"e}tes echinospora, and Iso{\"e}tes maritima from Hokkaido, Kamchatka, and Alaska regions. The distinct species status of I. asiatica was also well supported in the combined chloroplast DNA phylogeny. Therefore, I. asiatica appear to represent example of gradual speciation due to spatial isolation of ancestral populations followed by genetic divergence. Our results also suggest that I. asiatica is probably not the ancestral diploid of the polyploids occurring in East Asia. {\textcopyright} The Botanical Society of Korea 2009.},
	keywords = {AFLP, Chloroplast DNA, I. asiatica, Isoetes, Molecular phylogeny, NrITS},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-71249137227\&partnerID=40\&md5=029dcd01eab0eacc2d8c97b3953cb1ba},
	author = {Kim, C. and Na, H.R. and Shin, H. and Choi, H.-K.}
}
