@article {161,
	title = {The ligule of isoetes lacustris: Ultrastructure, mucilage composition, and a possible pathway of secretion},
	journal = {Annals of Botany},
	volume = {49},
	year = {1982},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {1982///},
	pages = {569 - 584},
	abstract = {Mature ligules of Isoetes lacustris can be divided anatomically into three ultrastructurally different regions. First, the basal region contains large numbers of two types of protein bodies. Second, the cells of the sub-marginal region are characterized by a well developed Golgi apparatus closely associated with the tubular compartments of the endoplasmic reticulum. Third, the peripheral region consists of one to three layers of cells showing an extremely well developed rough asternal and smooth tubular endoplasmic reticulum (ER). The tubular ER compartments are frequently observed in close attachment to the plasmalemma. The outermost peripheral cells are covered with a mucilaginous layer. The dry matter in the mucilage consists of 49 per cent polysaccharides and 22 per cent proteins. The polysaccharide fraction, analysed by ion exchange chromato-graphy, consists mainly of glucose, arabinose, galactose and uronic acids. The protein fraction was analysed by SDS gel dectrophoresis and by high performance liquid-chromatographic separation of the amino acids. The analysis shows a protein pattern very similar to that of the peripheral ligule tissue. It is suggested, therefore, that the material of the external mucilage is secreted by the peripheral ligule cells. The secretional mechanism appears to be a direct release of polysaccharides and proteins by the tubular components of the ER. There is no indication of secretion via the Golgi apparatus. Because of its high activity in protein synthesis and secretion, it is suggested that the ligule is a vestigial structure, which, in extinct genera, might have functioned as a digestive organ. {\textcopyright} 1982 Annals of Botany Company.},
	keywords = {Endoplasmic reticulum, Isoetes lacustris, Ligule, Polysaccharide secretion, Protein secretion, Ultrastructure},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-0008468496\&partnerID=40\&md5=1f528bd1dc2bfcc71d9f4a8b7f66d660},
	author = {Kristen, U. and Liebezeit, G. and Biedermann, M.}
}
@article {67,
	title = {Genetic consequence of restricted habitat and population decline in endangered Isoetes sinensis (Isoetaceae)},
	journal = {Annals of Botany},
	volume = {96},
	year = {2005},
	note = {Cited By (since 1996):19Export Date: 11 November 2013Source: Scopus},
	month = {2005///},
	pages = {1265 - 1274},
	abstract = {{\textbullet} Background and Aims: Isoetes sinensis (Isoeteaceae) is a critically endangered aquatic quillwort in eastern China. Rapid decline of extant population size and local population extinction have occurred in recent years and have raised great concerns among conservationists. {\textbullet} Methods: Amplified fragment length polymorphisms (AFLPs) were used to investigate the genetic variation and population structure of seven extant populations of the species. {\textbullet} Key Results: Eight primer combinations produced a total of 343 unambiguous bands of which 210 (61{\textperiodcentered}2 \%) were polymorphic. Isoetes sinensis exhibited a high level of intra-population genetic diversity (H E) = 0{\textperiodcentered}118; hs = 0{\textperiodcentered}147; I = 0{\textperiodcentered}192; P = 35{\textperiodcentered}2 \%). The genetic variation within each of the populations was not positively correlated with their size, suggesting recent population decline, which is well in accordance with field data of demographic surveys. Moreover, a high degree of genetic differentiation (FST = 0{\textperiodcentered}535; G ST = 0{\textperiodcentered}608; θB = 0{\textperiodcentered}607) was detected among populations and no correlation was found between geographical and genetic distance, suggesting that populations were in disequilibrium of migration-drift. Genetic drift played a more important role than gene flow in the current population genetic structure of I. sinensis because migration of I. sinensis is predominantly water-mediated and habitat range was highly influenced by environment changes. {\textbullet} Conclusions: Genetic information obtained in the present study provides useful baseline data for formulating conservation strategies. Conservation management, including both reinforcement for in situ populations and ex situ conservation programmes should be carefully designed to avoid the potential risk of outbreeding depression by admixture of individuals from different regions. However, translocation within the same regional population should be considered as a measure of genetic enhancement to rehabilitate local populations. An ex situ conservation strategy for conserving all extant populations to maximize genomic representation of the species is also recommended. {\textcopyright} The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.},
	keywords = {AFLP, Genetic differentiation, Genetic diversity, Isoetes sinensis, Population structure, Pteridophyte},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27944462292\&partnerID=40\&md5=26404edf2320e666820f93f6dd369ebf},
	author = {Kang, M. and Ye, Q. and Huang, H.}
}
