@article {78,
	title = {Chromosome behavior during meiosis and development of spore mother cells in the Chinese quillwort Isoetes sinensis T. C. Palmer (Isoetaceae)},
	journal = {American Fern Journal},
	volume = {94},
	year = {2004},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {183 - 195},
	abstract = {Chromosome behavior during meiosis of the tetraploid Isoetes sinensis was observed to be similar to that of basic diploid Isoetes species. This observation was consistent with the previous report that polyploid species of Isoetes are allopolyploids. Meiosis was generally similar in both megaspore and microspore mother cells, but differences were observed. Due to the smaller size of the microspore mother cells, during prophase I, chromosomes are not as dispersed as those in megaspore mother cells. Cytomixis was observed in all stages of meiosis in microspore mother cells, but it was not seen at any stage of meiosis in megaspore mother cells. Cytomixis, lagging chromosomes, chromosome bridges, chromosome fragments, and micronuclei, observed during meiosis in I. sinensis, can affect sexual reproduction, but it does not appear to be a major cause of population decline in this species. The major cause of decline is habitat degradation from human disturbance. The few remaining populations of the Chinese quillwort are fragmented and the numbers of individuals contained in these populations dwindle as a result of habitat degradation. Conservation efforts are needed to save I. sinensis from extinction.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11444270683\&partnerID=40\&md5=e3b19a6dd117ccd20573e526dceb63bf},
	author = {He, Z. and Wang, H. and Li, J. and Ye, Q. and Taylor, W.C.}
}
@article {79,
	title = {Isoetes fluitans sp. nov.: The identity of Spanish plants of {\textquoteright}I. longissimum{\textquoteright}},
	journal = {Botanical Journal of the Linnean Society},
	volume = {146},
	year = {2004},
	note = {Cited By (since 1996):3Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {231 - 236},
	abstract = {Recent revision of North African specimens of Isoetes velata A. Braun and the closely related taxon l. longissimum Bory, together with Spanish material conventionally designated I. longissimum, suggests that the Spanish specimens constitute a new species, I. fluitans. This is described and illustrated. The North African taxon I. longissimum is probably not specifically distinct from I. velata. {\textcopyright} 2004 The Linnean Society of London.},
	keywords = {aquatic plant, Endemism, Iberian peninsula, Isoetaceae, taxonomy, Threatened plant},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-6344262161\&partnerID=40\&md5=621cece2b24256d4cab656a85119d395},
	author = {Romero, M.I. and Amigo, J. and Ramil, P.}
}
@article {80,
	title = {Phylogenetic relationships of Iso{\"e}tes (Iso{\"e}taceae) in China as revealed by nucleotide sequences of the nuclear ribosomal ITS region and the second intron of a LEAFY homolog},
	journal = {American Fern Journal},
	volume = {94},
	year = {2004},
	note = {Cited By (since 1996):17Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {196 - 205},
	abstract = {Iso{\"e}tes is an ancient lycopod lineage with a highly conserved morphology that provides few morphological characters to resolve the phylogeny of its species. Species appear to have evolved by divergence and allopolyploidy. The basic diploids I. hypsophila, I. taiwanensis, and I. yunguiensis and the tetraploid I. sinensis occur in China. Analysis of ITS sequences indicates that the Chinese Iso{\"e}tes species are part of an Australasian clade including I. brevicula from Western Australia and I. kirkii from New Zealand. Two distinct cloned sequences of the second intron of a LEAFY homolog were recovered from I. sinensis supporting the hypothesis that I. sinensis is an allotetraploid. One of the I. sinensis cloned sequences was similar to the I. taiwanensis sequence and the other cloned sequence was similar to the I. yunguiensis sequence identifying I. taiwanensis and I. yunguiensis as the likely parents of I. sinensis. Other cloned sequences recovered from I. sinensis were recombined parts of the two distinct sequences. Morphological evidence supporting an allotetraploid origin of I. sinensis is found in its larger microspore size and intermediate megaspore texture compared to I. taiwanensis, and I. yunguiensis.},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11444262662\&partnerID=40\&md5=7b7a2b568e139bee1b60bd62836ff6ef},
	author = {Taylor, W.C. and Lekschas, A.R. and Qing, F.W. and Liu, X. and Napier, N.S. and Hoot, S.B.}
}
@article {81,
	title = {Regulation of root anaerobiosis and carbon translocation by light and root aeration in Isoetes alpinus},
	journal = {Plant, Cell and Environment},
	volume = {27},
	year = {2004},
	note = {Cited By (since 1996):15Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {1102 - 1111},
	abstract = {The activity of alcohol dehydrogenase (ADH) was measured in corms and roots of the submerged freshwater macrophyte Isoetes alpinus Kirk. growing in situ, and related to its capacity for internal oxygen transport and to carbohydrate translocation. ADH activity was present in roots but not corms at uniform activity (0.15-0.35 {\texttimes} 10-6 mol g-1 fresh weight s-1) over the entire plant depth range (3-7 m depth), and was intermediate to that developed in excised roots after 1-week exposure to either dissolved oxygen at air-saturation or to anoxia. Responses of photosynthesis and root oxygen release to light intensity confirmed that shoot-to-root oxygen transport saturated at similar light intensities to photosynthetic oxygen evolution, but was positive in the dark and at irradiances below the compensation point for photosynthesis, due to contributions to transport by oxygen diffusion from the external medium. Transport of 14C-labelled photo-assimilates to roots nevertheless ceased when intact plants were exposed to a combination of leaf darkness and root external anoxia, even when high 14C concentrations were present in shoots, but remained high when the roots were provided with external oxygen. The lack of any control over permeability of the root surface to gases in this species suggested that ADH activity and reduced translocation is most likely caused by development of hypoxic tissues in the apical tissue. These results suggest that reductions in ambient light intensity may have indirect effects on I. alpinus viability by increasing the degree of root hypoxia and impairing carbon partitioning.},
	keywords = {Alcohol dehydrogenase, Anoxia, Aquatic macrophytes, Assimilates, Lacunae, Oxygen transport, Photosynthesis, Respiration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-4544301436\&partnerID=40\&md5=3f174fe3d7839650597cfe52d3369b91},
	author = {Sorrell, B.K.}
}
@article {82,
	title = {Experimental management of Mediterranean temporary pools for conservation of the rare quillwort Isoetes setacea},
	journal = {Biological Conservation},
	volume = {118},
	year = {2004},
	note = {Cited By (since 1996):23Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {675 - 684},
	abstract = {Mediterranean temporary pools represent unique species-rich habitats with many rare and endangered species. The Roque-Haute Natural Reserve is one of the few sites in France with Isoetes setacea Lam., a very rare Mediterranean quillwort, considered as a keystone species determining a priority habitat for the European Union. Land uses changes have favoured the invasion of the pools by several shrub and tree species (e.g., Ulmus minor, Fraxinus angustifolia subsp. oxycarpa). Clearing shrubs in an experimental area of one pool resulted in the increase of I. setacea as compared to control, combining a higher cover in previously occupied quadrats and more quadrats occupied. Litter accumulation had a negative effect on the population of Isoetes but this effect was detectable only in the cleared area. Light attenuation was shown experimentally to be detrimental to the vegetative and regenerative production of I. setacea. Clearing of the shrubs and removal of the litter led to an increase in the I. setacea populations and therefore appears to be an effective technique for restoring these plant communities invaded by shrubs. Long-term shrub control should be ensured by reintroducing sheep grazing in order to enhance the sustainable conservation of this oligotrophic habitat. {\textcopyright} 2003 Elsevier Ltd. All rights reserved.},
	keywords = {Artificial shading, Endangered species, Grazing, Isoetes setacea, Light reduction, Litter effect, Mediterranean seasonal pools, Sexual reproduction, Spore-bank, Vegetative growth},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2042522718\&partnerID=40\&md5=9b3e2127f0abc60df6e5407fa4e555ec},
	author = {Rhazi, M. and Grillas, P. and Charpentier, A. and M{\'e}dail, F.}
}
@article {83,
	title = {Water velocity and irradiance effects on internal transport and metabolism of methane in submerged Isoetes alpinus and Potamogeton crispus},
	journal = {Aquatic Botany},
	volume = {79},
	year = {2004},
	note = {Cited By (since 1996):3Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {189 - 202},
	abstract = {Submerged aquatic macrophytes are important methane (CH4) transport pathways, but little is known about how water velocity and illumination affect plant CH4 transport. We studied CH4 transport in vitro in fully-submerged plants of two aquatic macrophytes, Isoetes alpinus and Potamogeton crispus, comparing transport between two water velocities (0.5 and 90 mm s-1) and light versus dark treatments at the shoots. In I. alpinus, CH4 taken up by the roots accumulated in the lacunar system of the leaves at concentrations \&gt;40 μmol l-1 at both flow rates, but there was no release from the shoots to the water at flow = 0.5 mm s-1, whereas at 90 mm s-1 release from the shoots to the water was always \&gt;0.15 μmol (CH4 g-1 dry wt. h-1). Uptake of CH4 by the roots was 1.5 times greater at the higher than the lower water velocity. In P. crispus, a gradient in lacunar [CH4] developed from the base to the apex of the shoot and was steeper at the higher than the lower water velocity. CH4 release rates from the shoots were \&lt;0.1 μmol (CH4 g-1 dry wt. h-1) at both water velocities in this species, due to CH 4 consumption by epiphytic methanotrophic bacteria. Irradiance and its associated root aeration had no effect on CH4 transport in I. alpinus, but decreased root to shoot CH4 transport and release more than 10-fold in P. crispus, apparently by alleviating effects of hypoxia on the root-associated methanotrophic bacteria. Our data support the view that plant-associated methanotrophs are important CH4 sinks in freshwater habitats, and demonstrate the importance of flow velocity in the CH4 dynamics of submerged macrophytes. {\textcopyright} 2004 Elsevier B.V. All rights reserved.},
	keywords = {CH4, Diffusion, Lacunar system, O2, Submerged macrophytes},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-2642545092\&partnerID=40\&md5=9d9974a9e87e34dbe2225f98137ef822},
	author = {Sorrell, B.K. and Downes, M.T.}
}
@article {84,
	title = {Revealing unknown or extinct lineages within Iso{\"e}tes (Iso{\"e}taceae) using DNA sequences from hybrids},
	journal = {American Journal of Botany},
	volume = {91},
	year = {2004},
	note = {Cited By (since 1996):25Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {899 - 904},
	abstract = {Iso{\"e}tes, a heterosporous lycopod with a fossil record dating back to the Paleozoic, has numerous putative allopolyploids (resulting from hybridization events coupled with doubling of chromosome number). By using the highly variable nucleotide sequences from the second intron of a LFY homologue in Iso{\"e}tes, species could be delimited and hybrid origins determined. The data suggest that reticulate evolution is both common and complex within a more derived species complex of Iso{\"e}tes. Sequences of identifiable parentage and sequences that are unlike any diploid species known were recovered, leading to the conclusion that one or both of the putative parents have not yet been discovered or are extinct. A range of observations concerning allopolyploid speciation were categorized as follows: (1) verification of previous hypotheses regarding parentage (e.g., I. riparia, I. appalachiana), (2) determination that two morphologically distinct allotetraploid species can share the same parentage (I. azorica and I. acadiensis), (3) recognition of a cryptic allotetraploid species, indicated by the presence of different parental genomes (I. "appalachiana" from Florida), and (4) identification of allotetraploid species with one or two unknown parents (e.g., I. tuckermanii, I. acadiensis, I. azorica, and I. hyemalis). Some sequences from diploid species are remarkably uniform among populations (e.g., I. echinospora from various locations in North America, Iceland, and Wales), while others are variable at the subspecies level (e.g., northern and southern populations within I. engelmannii).},
	keywords = {Allopolyploidy, Hybrid origins, Isoetes, LEAFY intron},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-11444256350\&partnerID=40\&md5=420a4ad06820ce82f60d7a70e7db9dec},
	author = {Hoot, S.B. and Napier, N.S. and Taylor, W.C.}
}
@article {85,
	title = {Non-destructive assessing and monitoring of populations of Isoetes lacustris L},
	journal = {Limnologica},
	volume = {34},
	year = {2004},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2004///},
	pages = {147 - 153},
	abstract = {Extensive studies of Isoetes lacustris, performed throughout Europe, resulted in regressions between the number of leaves per rosette and (1) the number of spores per megasporangium, (2) length of seasonal growth, (3) Secchi depth and (4) water colour plus a relationship between pH and the number of spores per megasporangium. The evaluation of Isoetes populations requires measurements of the lake data Secchi depth, water colour, pH and conductivity, the establishment of climate data and leaf counts on 20-30 plants with the aid of SCUBA. The method may be applied to lakes in a range of situations, varying between lowland and highland, and between temperate and subarctic climate regions. The regressions are displayed graphically. The actual mean leaf number and corresponding megaspore number are compared with the numbers referred to in the environmental data. Consistent values point to the parameters which influence growth and vitality of the quillwort and to what extent, in a particular lake. Examples of evaluation and monitoring are given.},
	keywords = {Climate, Conductivity, Evaluation, Isoetes lacustris, Leaf number, Light availability, Megaspore number, pH value},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-17944391690\&partnerID=40\&md5=bc81c2908849af47d9ff68a2cff5dbc9},
	author = {V{\"o}ge, M.}
}
