@article {38,
	title = {Systematic evaluation of iso{\"e}tes asiatica makino (iso{\"e}taceae) based on AFLP, nrITS, and chloroplast DNA sequences},
	journal = {Journal of Plant Biology},
	volume = {52},
	year = {2009},
	note = {Cited By (since 1996):2Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {501 - 510},
	abstract = {On the basis of amplified fragment length polymorphism (AFLP) and nucleotide sequence data from nuclear ribosomal internal transcribed spacer (nrITS) and three chloroplast DNA regions (rbcL, cpITS, and trnS-psbC spacer), we investigated the species delimitation and the evolutionary lineage of Iso{\"e}tes asiatica from Hokkaido, Japan. The neighbor-joining (NJ) dendrogram based on AFLP markers revealed the well-defined clusters (bootstrap value = 100\%) of I. asiatica. Results from the principal component analysis are largely congruent with those obtained in the NJ dendrogram. The maximum parsimony analysis, based on data from nrITS and three chloroplast DNA sequences, supported a monophyly of three species, I. asiatica, Iso{\"e}tes echinospora, and Iso{\"e}tes maritima from Hokkaido, Kamchatka, and Alaska regions. The distinct species status of I. asiatica was also well supported in the combined chloroplast DNA phylogeny. Therefore, I. asiatica appear to represent example of gradual speciation due to spatial isolation of ancestral populations followed by genetic divergence. Our results also suggest that I. asiatica is probably not the ancestral diploid of the polyploids occurring in East Asia. {\textcopyright} The Botanical Society of Korea 2009.},
	keywords = {AFLP, Chloroplast DNA, I. asiatica, Isoetes, Molecular phylogeny, NrITS},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-71249137227\&partnerID=40\&md5=029dcd01eab0eacc2d8c97b3953cb1ba},
	author = {Kim, C. and Na, H.R. and Shin, H. and Choi, H.-K.}
}
@article {41,
	title = {Competition in microcosm between a clonal plant species (Bolboschoenus maritimus) and a rare quillwort (Isoetes setacea) from Mediterranean temporary pools of southern France},
	journal = {Hydrobiologia},
	volume = {634},
	year = {2009},
	note = {Cited By (since 1996):5Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {115 - 124},
	abstract = {Bolboschoenus maritimus, a clonal species, is locally invasive in Mediterranean temporary pools where it threatens endangered rare plant species such as Isoetes setacea. The combination of management modifications (grazing) and of the progressive accumulation of fine sediments in the pools contributed to the establishment of competitive perennial plants such as B. maritimus. The competitive advantage of B. maritimus on I. setacea has been studied in controlled conditions. The goal of this experiment was to assess the role of environmental conditions in the output of the competition between Bolboschoenus and Isoetes, notably hydrology and soil richness. For this purpose, Isoetes was cultivated alone (three individuals/pot) and with Bolboschoenus (three individuals of both species). The experiment was run with five replicates on six types of sediment (gradient of richness in sand/silt/clay) combined with three hydrological treatments (flooded, wet. and dry). The competitive advantage of Bolboschoenus was measured as the ratio of the production of Isoetes in mixture versus monoculture. The results showed that Isoetes was always outcompeted by Bolboschoenus. However, the competitive advantage of Bolboschoenus on Isoetes, was more related to hydrology than to soil richness. The competitive advantage of Bolboschoenus was very high in wet and flooded conditions and very low in dry conditions. This situation may lead to the extinction, medium-term, of the populations of I. setacea. The introduction of ovine grazing or of cut back practices in temporary pools could reduce the B. maritimus biomass and help toward the conservation of I. setacea populations. {\textcopyright} Springer Science+Business Media B.V. 2009.},
	keywords = {Bolboschoenus maritimus, Competition, Hydrology, Isoetes setacea, Soil, Temporary pools},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-71549166625\&partnerID=40\&md5=75f2af6039d9ffd9ec5dc7303a7b1612},
	author = {Rhazi, M. and Grillas, P. and Rhazi, L. and Charpentier, A. and M{\'e}dail, F.}
}
@article {44,
	title = {Effects of aluminium toxicity and low pH on the early development of iso{\"e}tes echinospora},
	journal = {Preslia},
	volume = {81},
	year = {2009},
	note = {Cited By (since 1996):5Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {135 - 149},
	abstract = {A relict population of Iso{\"e}tes echinospora Durieu survived a thirty-year period of severe acidification and high concentrations of phytotoxic aluminium (Al) in Ple{\v s}n{\'e} Lake (Bohemian Forest, Czech Republic). The population consisted of only adult plants. Sporeling survival and age structure were examined during the population recovery in 2004-2008. Laboratory experiments were conducted to assess the effect of various pH values (4-8) and Al concentrations (0-1000 μgl -1) on sporeling development. The responses of the sporelings to the experimental treatments were evaluated and compared with those observed in the lake. The experiments showed that an Al concentration higher than 300 μgl- 1 , and high acidity (pH 4), inhibit sporeling growth, in particular resulted in a pronounced reduction in absorptive organs (macrogametophyte rhizoids, roots and root hairs). With increasing concentrations of Al and at pH 4, the ratio of the below-ground to above-ground sporeling biomass decreased to less than 1. The responses of the lake sporelings, rooting in the upper sediment layer, were similar to those exposed to 100-300 μgl -1 of Al in the laboratory, and reflected the Al toxicity of the lake water. The quillworts at Ple{\v s}n{\'e} Lake survived because adult plants can tolerate these adverse conditions and are very long-lived. The population recovered when the pH of the water increased to over 5 and the Al concentration decreased to below 300 μgl- 1 .},
	keywords = {Acidification, Demography, Iso{\"e}tes echinospora, Quillwort, Recovery, Reproduction, Sporeling},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-68249099192\&partnerID=40\&md5=9b6556ca288bc4b4849c6e259815eaca},
	author = {{\v C}tvrtl{\'\i}kov{\'a}, M. and Vrba, J. and Znachor, P. and Hekera, P.}
}
@article {45,
	title = {Genetic diversity and population structure of diploid and polyploid species of isoetes in east asia based on amplified fragment length polymorphism markers},
	journal = {International Journal of Plant Sciences},
	volume = {170},
	year = {2009},
	note = {Cited By (since 1996):5Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {496 - 504},
	abstract = {Isoetes L. is critically endangered in areas of East Asia, including Taiwan, Japan, and South Korea. The genetic diversity and the population structure of six Isoetes species from East Asia were evaluated by using amplified fragment length polymorphism (AFLP). Three AFLP-selective primer combinations generated a total 923 amplification products, of which 919 (99.6\%) were polymorphic. The levels of genetic diversity of two diploid species (I. taiwanensis [percentage of polymorphic loci (PPL) = 33.1\%-38.3\%] and I. asiatica [PPL = 49.0\%]) are higher than those of polyploid species (tetraploid: I. jejuensis [PPL = 9.3\%-29.3\%] and I. hallasanensis [PPL = 22.3\%]; hexaploid: I. coreana [PPL = 1.6\%-20.6\%] and I. japonica [PPL = 5.6\%-20.5\%]). Higher genetic differentiation was detected for populations of I. coreana (0B = 0.730) than for the diploid species I. taiwanensis (0B = 0.074). In addition, analysis of molecular variance showed that a large proportion (79.8\%) of genetic differentiation existed among population of I. coreana. However, 93.2\% of the total genetic variation was attributed to differentiation within populations of I. taiwanensis. A neighbor-joining dendrogram resulted in three clusters, one for I. asiatica, one for I. japonica, and one for the rest of the species, subdivided into two: (1) I. taiwanensis/I. jejuensis and (2) I. coreana/I. hallasanensis. Furthermore, individuals from each population of I. coreana and I. jejuensis were clustered into population-specific subclusters. The probable causes of the current pattern of genetic variation and population structure are discussed, and suggestions on the future protection of Isoetes species are also given. {\textcopyright} 2009 by The University of Chicago. All rights reserved.},
	keywords = {AFLP, Diploid, Genetic diversity, Isoetes, Polyploid, Population structure},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67649230633\&partnerID=40\&md5=794650fbe3653559e7cb90004dfa45c6},
	author = {Kim, C. and Shin, H. and Choi, H.-K.}
}
@article {47,
	title = {High allozyme diversity and unidirectional linear migration patterns within a population of tetraploid Isoetes sinensis, a rare and endangered pteridophyte},
	journal = {Aquatic Botany},
	volume = {90},
	year = {2009},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {52 - 58},
	abstract = {Isoetes sinensis (Isoetaceae), an aquatic quillwort which occurs only in two fragmented sites of China as an allotetraploid, is critically endangered. Genetic variation among eight subpopulations of I. sinensis was examined in the Xin{\textquoteright}an River (119{\textdegree}14'-15'E, 29{\textdegree}28'N) by using allozyme polymorphism. Eighteen loci of 10 enzyme systems were examined and used for the analysis of population genetic parameters. As expected for allotetraploids, fixed heterozygosity was found at four loci. A high level of genetic diversity was observed in the population, with mean number of alleles per loci of 1.8, and mean percentage of polymorphic loci of 55.6\%, which were much higher than the average values in fern species. The genetic variation within each subpopulation was not positively correlated with its size, which may be explained by high gene flow (Nm = 2.57), clonal reproduction and fixed heterozygosity of allopolyploid. The I. sinensis population contained high clonal diversity (PD = 0.39, D = 0.95), indicating the successful seedling recruitment of the population. Significant positive relationship was detected between clonal diversity and the size of subpopulation. Partitioning the genetic diversity indicated that 91.1\% of the genetic variation was within subpopulations and only 8.9\% existed among subpopulations. The migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by a source-sink model, but with unidirectional gene flow among subpopulations underlined by hydrochoric force. The results were then discussed in relation to both in situ and ex situ conservation efforts of the population. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Allotetraploid, Genetic diversity, Hydrochory, Isoetes, Riparian plant, Unidirectional migration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-54249105798\&partnerID=40\&md5=962f14606c468408a8b8d98333cd3292},
	author = {Chen, Y.-Y. and Yang, W. and Li, W. and Li, Z.-Z. and Huang, H.-W.}
}
