@article {72,
	title = {Genetic variation within the endangered quillwort Iso{\"e}tes hypsophila (Isoetaceae) in China as evidenced by ISSR analysis},
	journal = {Aquatic Botany},
	volume = {82},
	year = {2005},
	note = {Cited By (since 1996):21Export Date: 11 November 2013Source: Scopus},
	month = {2005///},
	pages = {89 - 98},
	abstract = {The genetic diversity of 56 individuals of Iso{\"e}tes hypsophila Hand.-Mazz. from China was investigated by ISSR. Twelve primers were screened from 65 primers, and a total of 119 DNA fragments were scored, of these, 82\% were polymorphic bands, which indicated that high levels of genetic variation existed in the natural populations. Genetic diversity varied greatly among populations with the percentage of polymorphic band (PPB) values ranging from 8 to 35\%. An analysis of molecular variance (AMOVA) was used to apportion the variation between regions, among populations within regions, and within populations. Results indicated that most of the variance (85\%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations, however, were only 5 and 10\%, respectively. In the among-population analysis, the larger part of genetic variation (77\%) resided among populations, and less (23\%) presented differences within populations. UPGMA cluster analysis showed that there was no distinct genetic differentiation between populations from Sichuan province. A number of causes including limited gene flow, genetic drift and inbreeding might have led to these observed genetic profiles of I. hypsophila. {\textcopyright} 2005 Elsevier B.V. All rights reserved.},
	keywords = {Conservation, Endangered, Genetic variation, Isoetes hypsophila, ISSR},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-20444420155\&partnerID=40\&md5=c58709593caeba345cf50c4fba7d6213},
	author = {Chen, J.-M. and Liu, X. and Wang, J.-Y. and Robert, G.W. and Wang, Q.-F.}
}
@article {52,
	title = {Chloroplast DNA phylogeography of the Chinese endemic alpine quillwort Isoetes hypsophila hand.-mazz. (isoetaceae)},
	journal = {International Journal of Plant Sciences},
	volume = {169},
	year = {2008},
	note = {Cited By (since 1996):6Export Date: 11 November 2013Source: Scopus},
	month = {2008///},
	pages = {792 - 798},
	abstract = {We examined chloroplast trnL intron sequence variation within Isoetes hypsophila, a Chinese endemic alpine quillwort species. Sequence data were obtained from 46 individuals in six populations throughout the natural distribution of the species. Sequences appeared to have neutral evolution (Tajima{\textquoteright}s criterion D = -1.66453, 0.1 \&gt; P \&gt; 0.05; Fu and Li{\textquoteright}s test D* = -1:92897, 0.1 \&gt; P \&gt; 0.05; F* = -2.17823, 0.1 \&gt; P \&gt; 0.05). Eleven haplotypes were identified in I. hypsophila. A relatively high level of haplotype diversity (h = 0.817) and a low level of nucleotide diversity (π = 0:0045) were detected in I. hypsophila. Indirect estimates of gene flow based on cpDNA variation revealed significant genetic differentiation between the ZD1 population in Yunnan Province and the populations from Sichuan Province. High genetic differentiation between Yunnan and Sichuan populations was consistently indicated by both hierarchical analyses of molecular variance (AMOVA; GST = 0.5011) and the structure of a neighbor-joining tree. From nested clade analysis, restricted gene flow with isolation by distance was inferred to be responsible for the current spatial genetic structure of I. hypsophila. {\textcopyright} 2008 by The University of Chicago. All rights reserved.},
	keywords = {Alpine quillwort, cpDNA trnL intron sequence, Isoetes hypsophila, Nested clade analysis, Phylogeography},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-47649125533\&partnerID=40\&md5=8f27b208869f24aef198146456c288ea},
	author = {Chen, J.-M. and Liu, F. and Gituru, W.R. and Wang, Q.-F.}
}
@article {30,
	title = {Genetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila Hand.-Mazz. revealed by AFLP markers},
	journal = {Plant Systematics and Evolution},
	volume = {290},
	year = {2010},
	note = {Cited By (since 1996):6Export Date: 11 November 2013Source: Scopus},
	month = {2010///},
	pages = {127 - 139},
	abstract = {Isoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered quillwort endemic to the Qinghai-Tibetan Plateau. Genetic variation and population structure of 12 I. hypsophila populations were examined by using amplified fragment length polymorphism (AFLP) markers. Eight primer pairs produced a total of 472 unambiguous bands, of which 229 (48.5\%) were polymorphic. Intra-population genetic variation of Isoetes hypsophila was low (PP = 14.9\%, HE = 0.039, hs = 0.084 and I = 0.061). The F statistics calculated by different approaches consistently revealed high genetic differentiation among populations, contributing approximately 50\% of total gene diversity. Four genetic groups corresponding to four geographic regions were detected, indicating significant geographic structure. Our results suggest that both ongoing evolutionary forces (e.g., inbreeding system, bottlenecks resulting from fluctuation of water level) and historical events (e.g., orogenic movements which reduce gene flow among populations, repeated population retreat, and recolonization during ice ages) may have contributed to the genetic structure of I. hypsophila. In conservation, attention should be paid to preserving every population, and intra-region translocations can currently be recommended. {\textcopyright} 2010 Springer-Verlag.},
	keywords = {AFLP, Aquatic quillwort, Genetic structure, Isoetes hypsophila, Qinghai-Tibetan Plateau},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78149498133\&partnerID=40\&md5=4c18cfb671d1d50e919e742e3bf86593},
	author = {Chen, Y.-Y. and Liao, L. and Li, W. and Li, Z.-Z.}
}
@article {8,
	title = {Genetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila (Isoetaceae) revealed by SSR analysis},
	journal = {Biochemical Systematics and Ecology},
	volume = {47},
	year = {2013},
	note = {Export Date: 11 November 2013Source: Scopus},
	month = {2013///},
	pages = {11 - 20},
	abstract = {Isoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered alpine quillwort endemic to the Qinghai-Tibetan Plateau (QTP). A total of 247 individuals of I. hypsophila from 11 populations were analyzed using ten pairs simple sequence repeats (SSR) markers. Seventy-seven alleles were recorded, with an average of 7.7 alleles per locus (ranging from 3 to 12) and the expected heterozygosity (HE) ranged from 0.392 to 0.536 (average 0.471). Clear population structure among I. hypsophila populations was found and the proportion of genetic differentiation among populations accounted for about 20\% of total genetic diversity (FST = 0.178; probability of allelic co-ancestory Fcoal = 0.238). This genetic structure was probably due to limited gene flow hindered by complex terrains, bottlenecks resulting from water fluctuation in habitats, and repeated population retreat and recolonization during the ice ages. In conservation, four management units corresponding to four geographic regions were suggested, and the populations from different management units with higher genetic variation should be the priority targets of both in situ and ex situ conservation. {\textcopyright} 2012 Elsevier Ltd.},
	keywords = {Conservation, Gene flow, Genetic structure, Isoetes hypsophila, Quillwort, Simple sequence repeat (SSR)},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84875089755\&partnerID=40\&md5=c1cc33debe532b0ac82fdd9916012f95},
	author = {Li, X.-L. and Li, S.-C. and Chu, H.-J. and Li, Z.-Z. and Chen, Y.-Y.}
}
@article {16,
	title = {Microsatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis},
	journal = {American Journal of Botany},
	volume = {99},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {e184 - e186},
	abstract = {Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di-and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. {\textcopyright} 2012 Botanical Society of America.},
	keywords = {Cross-amplification, Genetic diversity, Isoetaceae, Isoetes hypsophila, Isoetes sinensis, Microsatellite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84860573611\&partnerID=40\&md5=4de1802063e8ced0a9e32ab46966d215},
	author = {Li, Z. and Han, Q. and Chen, Y. and Li, W.}
}
