@article {57,
	title = {Genetic diversity in Isoetes yunguiensis, a rare and endangered endemic fern in China},
	journal = {Frontiers of Biology in China},
	volume = {2},
	year = {2007},
	note = {Cited By (since 1996):6Export Date: 11 November 2013Source: Scopus},
	month = {2007///},
	pages = {46 - 49},
	abstract = {Isoetes yunguiensis is an endangered and endemic fern in China. Field survey indicated that only one population and no more than 50 individuals occur in the wild. The genetic variation of 46 individuals from the population remaining at Pingba (Guizhou Province, China) was assessed by Random Amplified Polymorphic DNA (RAPD) finger-printing. Twelve primers were screened from sixty ten-bp arbitrary primers, and a total of 95 DNA fragments were scored. Of these, 62.1\% were polymorphic loci, which indicated that high level genetic variation existed in the natural population. The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I. yunguiensis population. However, with the continuing decrease of population size, the genetic diversity will gradually be lost. We suggest that the materials from the extant population should be used for re-establishment of the populations. {\textcopyright} 2007 Higher Education Press and Springer-Verlag.},
	keywords = {Endemic fern, Genetic diversity, Isoetes, Random amplified polymorphic DNA (RAPD), Rare and endangered},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-33847396236\&partnerID=40\&md5=ee6f56a9a5fcf2d93d7218f43862d556},
	author = {Chen, J. and Gituru, W.R. and Liu, X. and Wang, Q.}
}
@article {45,
	title = {Genetic diversity and population structure of diploid and polyploid species of isoetes in east asia based on amplified fragment length polymorphism markers},
	journal = {International Journal of Plant Sciences},
	volume = {170},
	year = {2009},
	note = {Cited By (since 1996):5Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {496 - 504},
	abstract = {Isoetes L. is critically endangered in areas of East Asia, including Taiwan, Japan, and South Korea. The genetic diversity and the population structure of six Isoetes species from East Asia were evaluated by using amplified fragment length polymorphism (AFLP). Three AFLP-selective primer combinations generated a total 923 amplification products, of which 919 (99.6\%) were polymorphic. The levels of genetic diversity of two diploid species (I. taiwanensis [percentage of polymorphic loci (PPL) = 33.1\%-38.3\%] and I. asiatica [PPL = 49.0\%]) are higher than those of polyploid species (tetraploid: I. jejuensis [PPL = 9.3\%-29.3\%] and I. hallasanensis [PPL = 22.3\%]; hexaploid: I. coreana [PPL = 1.6\%-20.6\%] and I. japonica [PPL = 5.6\%-20.5\%]). Higher genetic differentiation was detected for populations of I. coreana (0B = 0.730) than for the diploid species I. taiwanensis (0B = 0.074). In addition, analysis of molecular variance showed that a large proportion (79.8\%) of genetic differentiation existed among population of I. coreana. However, 93.2\% of the total genetic variation was attributed to differentiation within populations of I. taiwanensis. A neighbor-joining dendrogram resulted in three clusters, one for I. asiatica, one for I. japonica, and one for the rest of the species, subdivided into two: (1) I. taiwanensis/I. jejuensis and (2) I. coreana/I. hallasanensis. Furthermore, individuals from each population of I. coreana and I. jejuensis were clustered into population-specific subclusters. The probable causes of the current pattern of genetic variation and population structure are discussed, and suggestions on the future protection of Isoetes species are also given. {\textcopyright} 2009 by The University of Chicago. All rights reserved.},
	keywords = {AFLP, Diploid, Genetic diversity, Isoetes, Polyploid, Population structure},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-67649230633\&partnerID=40\&md5=794650fbe3653559e7cb90004dfa45c6},
	author = {Kim, C. and Shin, H. and Choi, H.-K.}
}
@article {47,
	title = {High allozyme diversity and unidirectional linear migration patterns within a population of tetraploid Isoetes sinensis, a rare and endangered pteridophyte},
	journal = {Aquatic Botany},
	volume = {90},
	year = {2009},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {52 - 58},
	abstract = {Isoetes sinensis (Isoetaceae), an aquatic quillwort which occurs only in two fragmented sites of China as an allotetraploid, is critically endangered. Genetic variation among eight subpopulations of I. sinensis was examined in the Xin{\textquoteright}an River (119{\textdegree}14'-15'E, 29{\textdegree}28'N) by using allozyme polymorphism. Eighteen loci of 10 enzyme systems were examined and used for the analysis of population genetic parameters. As expected for allotetraploids, fixed heterozygosity was found at four loci. A high level of genetic diversity was observed in the population, with mean number of alleles per loci of 1.8, and mean percentage of polymorphic loci of 55.6\%, which were much higher than the average values in fern species. The genetic variation within each subpopulation was not positively correlated with its size, which may be explained by high gene flow (Nm = 2.57), clonal reproduction and fixed heterozygosity of allopolyploid. The I. sinensis population contained high clonal diversity (PD = 0.39, D = 0.95), indicating the successful seedling recruitment of the population. Significant positive relationship was detected between clonal diversity and the size of subpopulation. Partitioning the genetic diversity indicated that 91.1\% of the genetic variation was within subpopulations and only 8.9\% existed among subpopulations. The migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by a source-sink model, but with unidirectional gene flow among subpopulations underlined by hydrochoric force. The results were then discussed in relation to both in situ and ex situ conservation efforts of the population. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Allotetraploid, Genetic diversity, Hydrochory, Isoetes, Riparian plant, Unidirectional migration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-54249105798\&partnerID=40\&md5=962f14606c468408a8b8d98333cd3292},
	author = {Chen, Y.-Y. and Yang, W. and Li, W. and Li, Z.-Z. and Huang, H.-W.}
}
@article {21,
	title = {Microsatellite analysis reveals the genetic structure and gene flow of the aquatic quillwort Isoetes sinensis, a critically endangered species in China},
	journal = {Aquatic Botany},
	volume = {96},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {52 - 57},
	abstract = {Isoetes sinensis is a critically endangered aquatic quillwort, occurring in two fragmented sites of China (Xiuning county of Anhui; Jiande county of Zhejiang). During a five-year period (2004-2009), the areas and sizes of the two populations diminished dramatically due to intensive human activities. Genetic structure of the species was investigated using simple sequence repeat makers (SSRs). For seven nuclear microsatellites, high levels of genetic diversity were found within populations (H E=0.324-0.447). Strong genetic differentiation was detected between populations (G ST=0.376), while weak genetic differentiation was found within populations (G ST=0.026-0.080). Notably, in contrast to the source-sink model suggested by previous study (Chen et al., 2009), the migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by the linear symmetrical non-adjacent flow model (LSNF), which indicates that intensive human activities of recent years have greatly affected the gene exchange pattern among I. sinensis subpopulations. {\textcopyright} 2011 Elsevier B.V.},
	keywords = {Allotetraploid, Genetic diversity, Isoetes, Migration pattern, Riparian plant, Stepping-stone model},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84855293635\&partnerID=40\&md5=824e647a2989ecda4486a69d98cdaac2},
	author = {Chen, Y.-Y. and Kong, D.-R. and Huang, C.-H. and Xu, Y.-X. and Li, Z.-Z.}
}
