@article {67,
	title = {Genetic consequence of restricted habitat and population decline in endangered Isoetes sinensis (Isoetaceae)},
	journal = {Annals of Botany},
	volume = {96},
	year = {2005},
	note = {Cited By (since 1996):19Export Date: 11 November 2013Source: Scopus},
	month = {2005///},
	pages = {1265 - 1274},
	abstract = {{\textbullet} Background and Aims: Isoetes sinensis (Isoeteaceae) is a critically endangered aquatic quillwort in eastern China. Rapid decline of extant population size and local population extinction have occurred in recent years and have raised great concerns among conservationists. {\textbullet} Methods: Amplified fragment length polymorphisms (AFLPs) were used to investigate the genetic variation and population structure of seven extant populations of the species. {\textbullet} Key Results: Eight primer combinations produced a total of 343 unambiguous bands of which 210 (61{\textperiodcentered}2 \%) were polymorphic. Isoetes sinensis exhibited a high level of intra-population genetic diversity (H E) = 0{\textperiodcentered}118; hs = 0{\textperiodcentered}147; I = 0{\textperiodcentered}192; P = 35{\textperiodcentered}2 \%). The genetic variation within each of the populations was not positively correlated with their size, suggesting recent population decline, which is well in accordance with field data of demographic surveys. Moreover, a high degree of genetic differentiation (FST = 0{\textperiodcentered}535; G ST = 0{\textperiodcentered}608; θB = 0{\textperiodcentered}607) was detected among populations and no correlation was found between geographical and genetic distance, suggesting that populations were in disequilibrium of migration-drift. Genetic drift played a more important role than gene flow in the current population genetic structure of I. sinensis because migration of I. sinensis is predominantly water-mediated and habitat range was highly influenced by environment changes. {\textbullet} Conclusions: Genetic information obtained in the present study provides useful baseline data for formulating conservation strategies. Conservation management, including both reinforcement for in situ populations and ex situ conservation programmes should be carefully designed to avoid the potential risk of outbreeding depression by admixture of individuals from different regions. However, translocation within the same regional population should be considered as a measure of genetic enhancement to rehabilitate local populations. An ex situ conservation strategy for conserving all extant populations to maximize genomic representation of the species is also recommended. {\textcopyright} The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.},
	keywords = {AFLP, Genetic differentiation, Genetic diversity, Isoetes sinensis, Population structure, Pteridophyte},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-27944462292\&partnerID=40\&md5=26404edf2320e666820f93f6dd369ebf},
	author = {Kang, M. and Ye, Q. and Huang, H.}
}
@article {16,
	title = {Microsatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis},
	journal = {American Journal of Botany},
	volume = {99},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {e184 - e186},
	abstract = {Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di-and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. {\textcopyright} 2012 Botanical Society of America.},
	keywords = {Cross-amplification, Genetic diversity, Isoetaceae, Isoetes hypsophila, Isoetes sinensis, Microsatellite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84860573611\&partnerID=40\&md5=4de1802063e8ced0a9e32ab46966d215},
	author = {Li, Z. and Han, Q. and Chen, Y. and Li, W.}
}
