@article {47,
	title = {High allozyme diversity and unidirectional linear migration patterns within a population of tetraploid Isoetes sinensis, a rare and endangered pteridophyte},
	journal = {Aquatic Botany},
	volume = {90},
	year = {2009},
	note = {Cited By (since 1996):7Export Date: 11 November 2013Source: Scopus},
	month = {2009///},
	pages = {52 - 58},
	abstract = {Isoetes sinensis (Isoetaceae), an aquatic quillwort which occurs only in two fragmented sites of China as an allotetraploid, is critically endangered. Genetic variation among eight subpopulations of I. sinensis was examined in the Xin{\textquoteright}an River (119{\textdegree}14'-15'E, 29{\textdegree}28'N) by using allozyme polymorphism. Eighteen loci of 10 enzyme systems were examined and used for the analysis of population genetic parameters. As expected for allotetraploids, fixed heterozygosity was found at four loci. A high level of genetic diversity was observed in the population, with mean number of alleles per loci of 1.8, and mean percentage of polymorphic loci of 55.6\%, which were much higher than the average values in fern species. The genetic variation within each subpopulation was not positively correlated with its size, which may be explained by high gene flow (Nm = 2.57), clonal reproduction and fixed heterozygosity of allopolyploid. The I. sinensis population contained high clonal diversity (PD = 0.39, D = 0.95), indicating the successful seedling recruitment of the population. Significant positive relationship was detected between clonal diversity and the size of subpopulation. Partitioning the genetic diversity indicated that 91.1\% of the genetic variation was within subpopulations and only 8.9\% existed among subpopulations. The migration pattern of I. sinensis along the Xin{\textquoteright}an River is best explained by a source-sink model, but with unidirectional gene flow among subpopulations underlined by hydrochoric force. The results were then discussed in relation to both in situ and ex situ conservation efforts of the population. {\textcopyright} 2008 Elsevier B.V. All rights reserved.},
	keywords = {Allotetraploid, Genetic diversity, Hydrochory, Isoetes, Riparian plant, Unidirectional migration},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-54249105798\&partnerID=40\&md5=962f14606c468408a8b8d98333cd3292},
	author = {Chen, Y.-Y. and Yang, W. and Li, W. and Li, Z.-Z. and Huang, H.-W.}
}
@article {30,
	title = {Genetic diversity and population structure of the endangered alpine quillwort Isoetes hypsophila Hand.-Mazz. revealed by AFLP markers},
	journal = {Plant Systematics and Evolution},
	volume = {290},
	year = {2010},
	note = {Cited By (since 1996):6Export Date: 11 November 2013Source: Scopus},
	month = {2010///},
	pages = {127 - 139},
	abstract = {Isoetes hypsophila Hand.-Mazz. (Isoetaceae) is an endangered quillwort endemic to the Qinghai-Tibetan Plateau. Genetic variation and population structure of 12 I. hypsophila populations were examined by using amplified fragment length polymorphism (AFLP) markers. Eight primer pairs produced a total of 472 unambiguous bands, of which 229 (48.5\%) were polymorphic. Intra-population genetic variation of Isoetes hypsophila was low (PP = 14.9\%, HE = 0.039, hs = 0.084 and I = 0.061). The F statistics calculated by different approaches consistently revealed high genetic differentiation among populations, contributing approximately 50\% of total gene diversity. Four genetic groups corresponding to four geographic regions were detected, indicating significant geographic structure. Our results suggest that both ongoing evolutionary forces (e.g., inbreeding system, bottlenecks resulting from fluctuation of water level) and historical events (e.g., orogenic movements which reduce gene flow among populations, repeated population retreat, and recolonization during ice ages) may have contributed to the genetic structure of I. hypsophila. In conservation, attention should be paid to preserving every population, and intra-region translocations can currently be recommended. {\textcopyright} 2010 Springer-Verlag.},
	keywords = {AFLP, Aquatic quillwort, Genetic structure, Isoetes hypsophila, Qinghai-Tibetan Plateau},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-78149498133\&partnerID=40\&md5=4c18cfb671d1d50e919e742e3bf86593},
	author = {Chen, Y.-Y. and Liao, L. and Li, W. and Li, Z.-Z.}
}
@article {16,
	title = {Microsatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis},
	journal = {American Journal of Botany},
	volume = {99},
	year = {2012},
	note = {Cited By (since 1996):1Export Date: 11 November 2013Source: Scopus},
	month = {2012///},
	pages = {e184 - e186},
	abstract = {Premise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di-and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. {\textcopyright} 2012 Botanical Society of America.},
	keywords = {Cross-amplification, Genetic diversity, Isoetaceae, Isoetes hypsophila, Isoetes sinensis, Microsatellite},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-84860573611\&partnerID=40\&md5=4de1802063e8ced0a9e32ab46966d215},
	author = {Li, Z. and Han, Q. and Chen, Y. and Li, W.}
}
