01460nas a2200265   4500008004100000245007800041210006900119260001200188300001400200490000700214520065700221653001600878653001200894653001800906653001000924653002700934653001400961653001100975653001600986100001701002700002101019700001701040700002001057856011701077       2005                        eng d00aTwo new species of the genus Isoetes L. (Isoetaceae-Lycopsida) from India0 aTwo new species of the genus Isoetes L IsoetaceaeLycopsida from   c2005///  a109 - 1160 v543 aTwo new, primitive quillwort species, I. udupiensis and I. divyadarshanii, are described from peninsular India. Cytologically both species are diploid and unique for India in having 22 chromosomes without fragments. Megaspores of both species are monomorphic, whereas in the previously described species, they are di-to polymorphic. Isoetes udupiensis is unique in having deltoid-shaped megaspores and dominant microspory. The similarity in morphology of both species to subgenus Euphyllum and to the remaining Indian species of subgenus Isoetes, suggests that they may be connecting links between primitive and derived forms within the genus in India.10aChromosomes10aDiploid10aI. udupiensis10aIndia10aIsoetes divyadarshanii10aPhylogeny10aSpores10aSystematics1 aShukla, P.K.1 aSrivastava, G.K.1 aShukla, S.K.1 aRajagopal, P.K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-15944372672&partnerID=40&md5=cad8ccfe64fc2bc9f7b174901a9b815302543nas a2200229   4500008004100000245016000041210006900201260001200270300001400282490000800296520175700304653000902061653001202070653002202082653001202104653001402116653002502130100001202155700001302167700001602180856011702196       2009                        eng d00aGenetic diversity and population structure of diploid and polyploid species of isoetes in east asia based on amplified fragment length polymorphism markers0 aGenetic diversity and population structure of diploid and polypl  c2009///  a496 - 5040 v1703 aIsoetes L. is critically endangered in areas of East Asia, including Taiwan, Japan, and South Korea. The genetic diversity and the population structure of six Isoetes species from East Asia were evaluated by using amplified fragment length polymorphism (AFLP). Three AFLP-selective primer combinations generated a total 923 amplification products, of which 919 (99.6%) were polymorphic. The levels of genetic diversity of two diploid species (I. taiwanensis [percentage of polymorphic loci (PPL) = 33.1%-38.3%] and I. asiatica [PPL = 49.0%]) are higher than those of polyploid species (tetraploid: I. jejuensis [PPL = 9.3%-29.3%] and I. hallasanensis [PPL = 22.3%]; hexaploid: I. coreana [PPL = 1.6%-20.6%] and I. japonica [PPL = 5.6%-20.5%]). Higher genetic differentiation was detected for populations of I. coreana (0B = 0.730) than for the diploid species I. taiwanensis (0B = 0.074). In addition, analysis of molecular variance showed that a large proportion (79.8%) of genetic differentiation existed among population of I. coreana. However, 93.2% of the total genetic variation was attributed to differentiation within populations of I. taiwanensis. A neighbor-joining dendrogram resulted in three clusters, one for I. asiatica, one for I. japonica, and one for the rest of the species, subdivided into two: (1) I. taiwanensis/I. jejuensis and (2) I. coreana/I. hallasanensis. Furthermore, individuals from each population of I. coreana and I. jejuensis were clustered into population-specific subclusters. The probable causes of the current pattern of genetic variation and population structure are discussed, and suggestions on the future protection of Isoetes species are also given. © 2009 by The University of Chicago. All rights reserved.10aAFLP10aDiploid10aGenetic diversity10aIsoetes10aPolyploid10aPopulation structure1 aKim, C.1 aShin, H.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-67649230633&partnerID=40&md5=794650fbe3653559e7cb90004dfa45c6