01798nas a2200229   4500008004100000245010400041210006900145260001200214300001400226490000800240520106000248653001401308653002201322653001801344653001201362653001401374653001701388100001701405700001701422700001201439856011701451       1994                        eng d00aBiosystematic studies on the genus Isoetes in Japan I. Variations of the somatic chromosome numbers0 aBiosystematic studies on the genus Isoetes in Japan I Variations  c1994///  a289 - 2970 v1073 aThe somatic chromosome number of three Japanese species of Isoetes, I. asiatica, I. japonica and I. sinensis, was determined in 199 individuals from 49 populations. The chromosome number of I. asiatica was 2 n=22, confirming previous reports. However, I. japonica and I. sinensis displayed a diversity in chromosome number. Six cytotypes, 2 n=66, 67, 77, 87, 88 and 89, were found in I. japonica; 2 n=67, 87, 88 and 89 are new counts in the genus Isoetes. The plants with 2 n=66 were the most frequent (72% of total individuals examined) and were distributed throughout Honshu and Shikoku. The plants with 2 n=88 occurred in western Honshu and a limited region in northeastern Honshu where the plants with 2 n=77 were also found. In contrast, four cytotypes, 2 n=44, 65, 66 and 68, were found in I. sinensis. The chromosome numbers of I. sinensis were reported here for the first time. The plants with 2 n=44 occurred only in Kyushu, while the plants with 2 n=66 were found throughout a large area of western Japan. © 1994 The Botanical Society of Japan.10aAneuploid10aChromosome number10aCytogeography10aIsoetes10aPolyploid10aPteridophyta1 aTakamiya, M.1 aWatanabe, M.1 aOno, K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-21844512580&partnerID=40&md5=5cb0d06bc24499fb20d76da2946e91ea02543nas a2200229   4500008004100000245016000041210006900201260001200270300001400282490000800296520175700304653000902061653001202070653002202082653001202104653001402116653002502130100001202155700001302167700001602180856011702196       2009                        eng d00aGenetic diversity and population structure of diploid and polyploid species of isoetes in east asia based on amplified fragment length polymorphism markers0 aGenetic diversity and population structure of diploid and polypl  c2009///  a496 - 5040 v1703 aIsoetes L. is critically endangered in areas of East Asia, including Taiwan, Japan, and South Korea. The genetic diversity and the population structure of six Isoetes species from East Asia were evaluated by using amplified fragment length polymorphism (AFLP). Three AFLP-selective primer combinations generated a total 923 amplification products, of which 919 (99.6%) were polymorphic. The levels of genetic diversity of two diploid species (I. taiwanensis [percentage of polymorphic loci (PPL) = 33.1%-38.3%] and I. asiatica [PPL = 49.0%]) are higher than those of polyploid species (tetraploid: I. jejuensis [PPL = 9.3%-29.3%] and I. hallasanensis [PPL = 22.3%]; hexaploid: I. coreana [PPL = 1.6%-20.6%] and I. japonica [PPL = 5.6%-20.5%]). Higher genetic differentiation was detected for populations of I. coreana (0B = 0.730) than for the diploid species I. taiwanensis (0B = 0.074). In addition, analysis of molecular variance showed that a large proportion (79.8%) of genetic differentiation existed among population of I. coreana. However, 93.2% of the total genetic variation was attributed to differentiation within populations of I. taiwanensis. A neighbor-joining dendrogram resulted in three clusters, one for I. asiatica, one for I. japonica, and one for the rest of the species, subdivided into two: (1) I. taiwanensis/I. jejuensis and (2) I. coreana/I. hallasanensis. Furthermore, individuals from each population of I. coreana and I. jejuensis were clustered into population-specific subclusters. The probable causes of the current pattern of genetic variation and population structure are discussed, and suggestions on the future protection of Isoetes species are also given. © 2009 by The University of Chicago. All rights reserved.10aAFLP10aDiploid10aGenetic diversity10aIsoetes10aPolyploid10aPopulation structure1 aKim, C.1 aShin, H.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-67649230633&partnerID=40&md5=794650fbe3653559e7cb90004dfa45c6