02381nas a2200253   4500008004100000245013000041210006900171260001200240300001600252490000700268520155900275653001501834653001101849653001201860653002101872653001201893653002301905653001501928653002201943100001701965700001701982700001201999856011602011       1996                        eng d00aBiosystematic studies on the genus Isoetes (Isoetaceae) in Japan. II. Meiotic behavior and reproductive mode of each cytotype0 aBiosystematic studies on the genus Isoetes Isoetaceae in Japan I  c1996///  a1309 - 13220 v833 aMeiotic behaviors and reproductive modes of Japanese Isoetes were studied. The hexaploid (2n = 66) and the octaploid (2n = 88) of I. japonica consistently formed 33 and 44 bivalents, respectively, at diakinesis and/or metaphase I in both micro- and megaspore mother cells. The tetraploid (2n = 44) of I. sinensis formed 22 bivalents and its hexaploid made 33 bivalents in both types of spore mother cells. At diakinesis and/or metaphase I of microspore mother cells in I. asiatica with 2n = 22, 11 bivalents were detected. Because behaviors of meiosis in all cytotypes mentioned above were quite regular and plants yielded normal-appearing spores, they should reproduce sexually. Aneuploids of I. japonica with 2n = 87 formed 43 bivalents and one univalent, and I. sinensis with 2n = 65 formed 32 bivalents and one univalent in microspore mother cells. Meiosis of both cytotypes was almost regular and yielded microspores of normal appearance. In the heptaploid (2n = 77) of I. japonica, a configuration of 22 bivalents and 33 univalents was detected in micro- and megaspore mother cells, and various irregularities were observed throughout the meiotic divisions. Therefore, the genomic formula of the heptaploid is symbolized as AABBCDE, and the heptaploid is a sterile F 1 hybrid between the hexaploid (AABBCC) and the octaploid (AABBDDEE) of I. japonica. Since diploid and even-numbered polyploids regularly formed bivalents and odd-numbered ones displayed irregularities, allopolyploidy should act as a significant speciation mechanism in this genus.10aChromosome10aHybrid10aIsoetes10amegasporogenesis10ameiosis10amicrosporogene sis10apolyplo id10areproductive mode1 aTakamiya, M.1 aWatanabe, M.1 aOno, K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0030475989&partnerID=40&md5=996182a1723b75d447e3a2137006f7bd