01578nas a2200121 4500008004100000245008400041210007000125300001200195490000700207520118400214100001401398856004401412 2014 eng d00aMonitoring the vitality of Isoëtes lacustris by using a non-destructive method0 aMonitoring the vitality of Isoëtes lacustris by using a nondestr a153-1580 v143 aThe aim of ongoing studies was to expand the non-destructive monitoring of Isoëtes lacustris (Vöge 2004) resulting in a description of the vitality and structure of the population. The influence of the environment on the development of the plant was the subject of extended studies carried out between 1995 and 2014 in more than 100 lakes throughout Europe. The environment was characterized through conductivity, pH and Secchi depth of the lake water and the accompanying submersed plant species, as well as the plant performance through the mean number of leaves of 20 sporogenous plants. The counting of the leaves was performed whilst diving. The vitality level was defined based on Vöge (2004) and related to the environmental conditions; these are referred to in the context. The inventory of the accompanying plant species may reflect a changing environment. The method is demonstrated through case examples. A highly significant correlation between the mean and the smallest number of leaves of sporogenous plants enables separating juvenile from adult plants. Finally, recent results show a relationship between the vitality level and the structure of the population.1 aVoege, M. uhttps://isoetes.myspecies.info/node/21200997nas a2200145 4500008004100000245007000041210006700111300001200178490000700190520055200197100001900749700002300768700001600791856004400807 2014 eng d00aIsoetes naipiana (Isoetaceae), a new species from southern Brazil0 aIsoetes naipiana Isoetaceae a new species from southern Brazil a393-3980 v443 aIsoetes naipiana (Isoetaceae), a new species from southern Brazil, is described, illustrated and compared to similar species. So far it is known only from the original locality in the open grasslands of the upper highlands in the NE region of the State of Rio Grande do Sul (Brazil). It is characterized by megaspores with an irregular reticulum on the surface, presenting deposition of thin parallel silica strands; laevigate microspores; and arcuate microphylls with shortly alate bases, forming a compact rosette around the erect bilobate corm.1 aWindisch, P.G.1 aLorscheitter, M.L.1 aNervo, M.H. uhttps://isoetes.myspecies.info/node/21100733nas a2200133 4500008004100000245006700041210006100108300000900169490000600178520033400184100001500518700002200533856004400555 2014 eng d00aTürkiye'nin Çimeğreltisi [Isoetes L. (Isoetaceae)] türleri0 aTürkiyenin Çimeğreltisi Isoetes L Isoetaceae türleri a1-130 v13 aIsoetes L. (Isoetaceae) species in Turkey. In this study, it was given detailed information about genus Isoetes L. (Isoetaceae) and its species which live in Turkey. It is also discussed about possible hybridations within Isoetes. A new identification key based on morphological characteristics was prepared for the Turkish taxa.1 aKeskin, M.1 aMusselman, L., J. uhttps://isoetes.myspecies.info/node/20800942nas a2200121 4500008004100000245006100041210005500102300001200157490000700169520057500176100002500751856004400776 2014 eng d00aThe family Isoëtaceae in Guerrero, Mexico: a new record0 afamily Isoëtaceae in Guerrero Mexico a new record a183-1880 v923 aDespite the intensive taxonomic studies on pteridophytes carried out in Mexico, particularly in the state of Guerrero, Isoëtaceae has not yet been reported in this state. Here the first report of Isoëtes pallida in the southeastern coastal plain near Acapulco is given and discussed. The finding of I. pallida in the state of Guerrero is the third record of this taxon in the country. Currently, the color and ornamentation of the sporangial wall are used to separate some species of Isoëtes, but the taxonomic value of these characteristics seems to be questionable.1 aVelazquez-Montes, E. uhttps://isoetes.myspecies.info/node/20702241nas a2200121 4500008004100000245008400041210007000125300001200195490000700207520185600214100000502070856004402075 2014 eng d00aThe effect of temperature on the phenology of germination of Isoëtes lacustris0 aeffect of temperature on the phenology of germination of Isoëtes a279-2920 v863 a

Isoëtes lacustris (quillwort) is an aquatic macrophyte commonly dominating oligotrophic softwater lakes in Europe. Reproductive ecology of a relic population of quillwort based on spore germination was studied in an acidified mountain lake in the Czech Republic. In a four-year experiment, we recorded temperature-related temporal changes in micro- and macrospore germination and sporeling establishment in (i) natural in situ conditions in Černé jezero lake and (ii) in the laboratory at various temperatures. Germination of both micro- and macrospores increased gradually over four consecutive growth seasons. Several annual cohorts of germinating macrospores born together in a sporangium indicate that spores remain viable for up to several years and the formation of a spore bank. Minimum temperature necessary for germination was lower for microspores (6 °C) than macrospores (12 °C) and this ensured the availability of spermatozoids for the fertilization of the long-living macrogamethophytes whenever they produced archegonia during growing season(s). Macrogametophyte development started between July and October and sporeling development always followed in the next or subsequent springs. Long germination and embryo development may limit reproduction in I. lacustris by making it sensitive to both episodic and chronic changes in the environment. The relatively high minimum temperature for macrospore germination may set general limits for the reproduction of I. lacustris in lakes, by constraining its distribution along latitudinal and altitudinal gradients, and to particular depths. The mean length of time when the temperature ( 12 °C) was high enough for the germination of I. lacustris in Černé jezero lake was 119 days and occurred during the period June to September in 2004–2011.

1 a uhttps://isoetes.myspecies.info/node/20401543nas a2200133 4500008004100000245003400041210003100075300001200106490000700118520120100125100002501326700001401351856004401365 2014 eng d00aEuro+Med-Checklist Notulae, 30 aEuroMedChecklist Notulae 3 a287-2990 v443 aThis is the third of a series of miscellaneous contributions, by various authors, where hitherto unpublished data relevant to both the Med-Checklist and the Euro+Med (or Sisyphus) projects are presented. This instalment deals with the families Isoetaceae; Amaranthaceae, Callitrichaceae, Campanulaceae, Caryophyllaceae, Chenopodiaceae, Compositae, Crassulaceae, Cruciferae, Geraniaceae, Leguminosae, Onagraceae, Polygonaceae, Resedaceae, Rosaceae, Salicaceae, Scrophulariaceae s.l. (incl. Orobanchaceae p.p., Plantaginaceae p.p. and Veronicaceae), Tamaricaceae; Cyperaceae, Gramineae, Liliaceae s.l. (incl. Hyacinthaceae) and Orchidaceae. It includes new country and area records, taxonomic and distributional considerations for taxa in Alternanthera, Bellardia, Bromopsis, Callitriche, Cardamine, Carex, Cotula, Draba, Dysphania, Echinops, Epilobium, Epipactis, Genista, Geranium, Kickxia, Lathyrus, Legousia, Lupinus, Mcneillia, Noccaea, Oligomeris, Persicaria, Pilosella, Pyracantha, Pyrus, Salix, Scrophularia, Sedum, Sempervivum, Suaeda and Tamarix, and the validation of names in Bellardia, Campanula, Draba, Isoetes, Mcneillia, Noccaea, Ornithogalum, Persicaria, Pilosella and Sempervivum.1 avon Raab-Straube, E.1 aRaus, Th. uhttps://isoetes.myspecies.info/node/20301275nas a2200145 4500008004100000245013800041210006900179300001200248490000800260520069500268100001400963700001900977700001600996856011701012 2014 eng d00aThe Isoetes longissima complex (Isoetaceae) in Italy: Observations on the morphology of spores and leaves, and taxonomic implications0 aIsoetes longissima complex Isoetaceae in Italy Observations on t a149-1560 v1743 aThe morphological variability of the Isoetes longissima complex in Italy has been analyzed, on the basis of selected herbarium specimens. Observations were made on spore ornamentation and size, number of leaves per plant and maximum leaf length, velum extension and alae width in the basal portion of leaves. The first count of chromosome number on plants from Italy is also made. On the basis of our observations, the five taxa here considered are attributed to two taxa, treated at the species level as follows: I. longissima (incl. I. velata and I. velata [unranked] sicula), and I. tiguliana (incl. I. dubia). Italian distribution of these species is also given. © 2014 Magnolia Press.1 aTroia, A.1 aRaimondo, F.M.1 aCampisi, P. uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84904159896&partnerID=40&md5=b6cf14d277eb08c8b30062a4218c63ea00640nas a2200193 4500008004100000022001400041245009500055210006900150300001400219490000800233653001500241653002600256100001400282700001700296700002400313700002500337700002100362856006300383 2014 eng d a0002-844400aHydrochemical characterization of a stand of the threatened endemic Isoëtes malinverniana0 aHydrochemical characterization of a stand of the threatened ende a241–2440 v10310aIsoetaceae10aIsoetes malinverniana1 aAbeli, T.1 aOrsenigo, S.1 aArdenghi, N., M. G.1 aLucassen, E.C.H.E.T.1 aSmolders, A.J.P. uhttp://www.bioone.org/doi/full/10.1640/0002-8444-103.4.19301031nas a2200217 4500008004100000022001400041245008200055210007000137300001100207490000800218520036900226653001500595653002600610653001600636100002700652700002600679700002100705700001700726700002600743856004400769 2014 eng d a0002-844400aIsoëtes viridimontana: A Previously Unrecognized Quillwort from Vermont, USA0 aIsoëtes viridimontana A Previously Unrecognized Quillwort from V a7–150 v1043 a

Isoetes viridimontana is described and illustrated. Plants of this species are currently known only from Haystack Pond, a small, acidic, oligotrophic lake in south central Vermont. Isoetes viridimontana is distinguished from other Isoetes species in northeastern North America by its size (less than 5 cm high) and granulate to rugulate megaspore texture.

10aIsoetaceae10aIsoetes viridimontana10aNew species1 aRosenthal, Michael, A.1 aRosenthal, Sharon, R.1 aJohnson, Gabriel1 aTaylor, Carl1 aZimmer, Elizabeth, A. uhttps://isoetes.myspecies.info/node/19902457nas a2200121 4500008004100000245006400041210006300105300001000168490000700178520201700185100001602202856011702218 2014 eng d00aHeterosporangium: A new category of sporangium in Lycopsida0 aHeterosporangium A new category of sporangium in Lycopsida a77-920 v563 aThis is being hypothesised that the heterospory to begin with, might have originated within one specific sporangium thereafter separate male and female sporangia would have evolved in due course of time. This new category of a sporangium, is hereby named as "heterosporangium", conceived to have been the cradle sporangium for the origin of heterospory. Such a sporangium may have possessed three types of spores, viz. microspores, megaspores and morphologically distinct spores possessing resemblances with spores of past lineages/fossils. Such a classical structure has been exemplified by a fundamental discovery of "intrasporangial heterospory" in 1968. This appears that this evolutionary phase could have followed the path of sequential evolution of isosporangium-anisosporangium-heterosporangium and finally, leading to "intersporangial heterospory" in the form of independent microsporangium and megasporangium. The independent sex differentiated sporangia must have had evolved by selective degeneration of spore mother cells in a differentiating young heterosporangium. In other words, a microsporangium should have evolved by the degeneration of megaspore mother cells and megasporangium by early degeneration of microspore mother cells. This selective degeneration must have been operative on account of some genetic mechanism as suggested by Bell in 1996 and later, demonstrated by our observations on epigenetic mechanisms suspecting mainly abrupt hypomethylation of DNA, to be responsible event as indicated in heterosporangia of Isoetes pantii Goswami et Arya. This paper exclusively establishes heterosporangium as a structure of rare evolutionary importance genetically inherent within the genome of the species observed consistently for more than five decades. Recent decline of such plants of I. × pantii does not lower down importance of heterosporangium because its physical presence can never be denied. The future of a species or an organ depends on operative modes of natural selection.1 aGoswami, H. uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84897648217&partnerID=40&md5=b534f6c64ae2778a73ed5149a525d6b501851nas a2200157 4500008004100000245008100041210006900122300001200191490000700203520130500210100001401515700001601529700001601545700001501561856011701576 2014 eng d00aGrowth regulation of Isoetes sinensis seedings planted in several substrates0 aGrowth regulation of Isoetes sinensis seedings planted in severa a117-1210 v123 aIsoetes sinensis is an ancient vascular plant in mires, and Chinese unique, critically endangered now and key wild plant for state protection. The seedlings of Isoetes sinensis were planted in the 6 kinds of substrates with different proportions of the dark brown soil and the sand from river, the vegetative and reproductive growth of the seedlings were studied in the green house of Key Laboratory of Botany of Heilongjiang Province during February 5 2010 to October 5 2012. Comparing the number of seedling leaves, the leaf lengths, formation of sporangium and condition of maturation under 6 kinds of substrates and 4 kinds of thicknesses of the dark brown soil, the suitable substrate for Isoetes sinensis growth was chosen. The resuits showed that the more proportion of the dark brown soil in the substrates, and the thicker of the dark brown soil, the more number of seedling leaves and the longer of leaf lengths of Isoetes sinensis, and the shorter time the sporangium grew and the spores matured. The higher proportion of the sand in the substrates would result in Isoetes sinensis seedling death or spore abortion, the environment with nutrient rich, little or no sand, shallow micro flow, high water quality, light transparent enough in the mires fits for the growth of Isoetes sinensis.1 aJiang, N.1 aXing, J.-J.1 aDing, G.-H.1 aLiu, B.-D. uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84896912419&partnerID=40&md5=77397ebb1a0c4bc35232b1a0e0d52d3102309nas a2200145 4500008004100000245015600041210006900197300001200266490000700278520171700285100001402002700001502016700001502031856011702046 2014 eng d00aA new insight into the phylogeny of vascular cryptogams with special reference to Selaginella and Isoetes inferred from nuclear ITS/5.8S rDNA sequences0 anew insight into the phylogeny of vascular cryptogams with speci a167-1740 v233 aIn order to provide a better understanding of the evolutionary history of vascular cryptogams, phylogenetic framework was developed based on ITS1, ITS2 and 5.8S rDNA sequences of 102 extant taxa of vascular cryptogams using Maximum Parsimony (MP) analysis. The analysis revealed high GC content in Isoetaceae (60.5 %) in comparison with Selaginellaceae (54.4 %) that was envisaged to be the result of variation in selection, mutational bias, and biased recombination-associated DNA repair within these two plant lineages during evolution. Transition/transversion ratio was observed to be 0.9 in Isoetaceae, 0.68 in Selaginellaceae and 0.57 among all the 102 taxa belonging to lycophytes and ferns. It is hypothesized that the lycophytes have been separated very early during evolution and therefore acquired independent line of evolution from the other plant lineages. Although Selaginellaceae and Isoetaceae are closely related ancient plant groups, pairwise sequence divergence of sampled taxa on the basis of transition and transversion, and disparity index values per site between sampled sequence pairs pointed towards the differential investment of natural selection process. These lead to high rate of nucleotide substitution within nuclear genome of Selaginellaceae with respect to Isoetaceae. MP phylogenetic tree identified Isoetes subinermis, Isoetes durieui and Salvia microphylla as separate group among the studied taxa due to high sequence variation within these species through the time of evolution. Our result interpreted the polyphyletic origin of ferns and provides valuable information regarding the lycophytes and their fern allies. © 2013 Society for Plant Biochemistry and Biotechnology.1 aSaha, J.a1 aGupta, K.b1 aGupta, B.a uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84883561608&partnerID=40&md5=94b9e48a0a9fe623154b9a728a9971f301214nas a2200229 4500008004100000020001400041245005800055210005400113260003300167300001000200490000800210520058500218653001800803653001900821653001500840653001800855653001300873653001700886100001400903700001600917856005100933 2014 eng d a1126-350400aA critical conspectus of Italian Isoetes (Isoetaceae)0 acritical conspectus of Italian Isoetes Isoetaceae bTaylor & Francisc2014/01/08 a13-200 v1483 a

The work on the Isoetaceae account for the Critical Flora of Italy has resulted in a new, synonymic inventory of Italian Isoetes taxa, based on herbarium studies, observation by scanning electron microscopy and critical re-examination of the previously published works. Ten species are listed. The names I. longissima and I. gymnocarpa are restored as correct, to replace I. velata and I. subinermis, respectively. Eighteen lectotypes, one epitype and one neotype are designated. A dichotomous key to Italian taxa is provided.

10aItalian flora10aLycopodiophyta10amorphology10aPteridophytes10ataxonomy10atypification1 aTroia, A.1 aGreuter, W. uhttp://dx.doi.org/10.1080/11263504.2013.87840902481nas a2200157 4500008004100000245012600041210006900167300001000236490000800246520189500254100001402149700001302163700001302176700001702189856011702206 2014 eng d00aMorphological and molecular characterization of a new record of Isoëtes coromandelina subsp. coromandelina from Cambodia0 aMorphological and molecular characterization of a new record of  a43-500 v3003 aPlants of a heterosporous fern, Isoëtes, were collected from a wetland in Pu Rodet Leu, Mondulkiri, Cambodia, in which the flora has not yet been well studied. The characteristics of this quillwort, for example, dimorphic megaspores and small tuberculate ornamentation, were congruent with those of Isoëtes coromandelina L. f. subsp. coromandelina. Unlike most Isoëtes endemic species, this subspecies has a wide geographical range across India and the neighboring regions, and another subspecies, I. coromandelina subsp. macrotuberculata Marsden, grows in Australia. We have provided detailed characteristics for the first record of an Isoëtes species in Cambodia. Moreover, megaspore morphological features and the sequence of the chloroplast trnL intron were investigated to verify infraspecific and regional variation within this species, using specimens from India (Rajasthan and Varanasi), Sri Lanka, and Australia. Dimorphic megaspores were observed in similar proportions in a megasporangium of the Cambodian plant; the large spherical type averaged 378.5 μm in height in 409 megaspores, and the small flattened type averaged 227.7 μm in height in 544 megaspores. Microspores were not observed. The Cambodian Isoëtes plant is a tetrapolyploid (2n = 44 + 1). The morphological characteristics of the Cambodian quillwort are closer to those of a plant from Varanasi, India, than to those of plants from other regions in terms of tubercle size on the megaspore surface and polyploidy of chromosomes. Three haplotypes of the trnL intron showed regional specificity according to the country. However, the phylogenetic monophyly of all the plants examined was supported among the phylogeny of the entire Isoëtes genus. This close molecular relationship indicates that a recent dispersal of I. coromandelina may be related to its present distribution. © 2013 Springer-Verlag Wien.1 aJung, J.a1 aRyu, Y.a1 aWon, H.b1 aChoi, H.-K.a uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84891663437&partnerID=40&md5=bef44b654531c54b87e278312422cef402550nas a2200193 4500008004100000022001400041245016400055210007000219300001000289490000800299520175900307653009702066100001802163700002602181700002702207700003102234700002002265856007102285 2014 eng d a0304-377000aGenetic diversity and population structure of Indian Isoëtes dixitei Shende based on amplified fragment length polymorphisms and intron sequences of \{LEAFY\}0 aGenetic diversity and population structure of Indian Isoëtes dix a1 - 70 v1133 aAbstract Isoëtes dixitei Shende is an endemic plant that grows in the Western Ghats, India. To evaluate the genetic diversity and the population structure of five I. dixitei populations from Maharashtra, we used amplified fragment length polymorphism (AFLP) markers and second intron of \{LEAFY\} sequences. Four AFLP-selective primer combinations generated 756 bands, of which 97% (735) from 154 individuals were polymorphic. In the analysis of the second intron of LEAFY, 103 sequences were generated and three types and 91 subtypes were obtained on considering the shared insertions/deletions and sequence similarity. The level of genetic diversity was high based on \{AFLP\} markers (mean Nei's unbiased expected heterozygosity [HE] = 0.207) and the second intron of \{LEAFY\} sequences (HE = 0.293). Estimated genetic differentiation among the populations was relatively low (θB = 0.082 on \{AFLP\} and 0.038 on LEAFY), compared with other Isoëtes species. A substantial amount of gene flow among populations was observed (Nem = 2.566 on \{AFLP\} and 2.855 on LEAFY). Moreover, a positive marginally significant correlation between genetic distances (ΦST) and geographical distances was detected by performing the Mantel test (r = 0.598, P = 0.062) based on \{AFLP\} markers. Isoëtes dixitei populations showed equilibrium of gene flow and genetic drift due to old-standing separation with sufficient gene flow. Two distinct groups were confirmed based on the genetic relationship: one contained two populations of Bhilar tableland (MB) and Wilson point (MW) while the other included three populations of Panchgani tableland (MP), Khingar tableland (MK), and Dandeghar tableland (MD).10aLEAFY1 aJung, Jongduk1 aSingh, Sarvesh, Kumar1 aPande, Harish, Chandra1 aSrivastava, Gopal, Krishna1 aChoi, Hong-Keun uhttp://www.sciencedirect.com/science/article/pii/S0304377013001472