00468nas a2200133   4500008004100000245005300041210005300094260001200147300001400159490000700173100002100180700001700201856011600218       1994                        eng d00aConfirmation of a hybrid Isoetes from New Jersey0 aConfirmation of a hybrid Isoetes from New Jersey  c1994///  a115 - 1200 v841 aMontgomery, J.D.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0028191624&partnerID=40&md5=023d53ceb2a6602cc358f568dbcb20c200871nas a2200157   4500008004100000245009600041210006900137260001200206300001200218490000700230520030700237100001800544700001800562700001700580856011600597       1994                        eng d00aIsoetes hyemalis sp. nov. (Isoetaceae): a new quillwort from the southeastern United States0 aIsoetes hyemalis sp nov Isoetaceae a new quillwort from the sout  c1994///  a12 - 210 v593 aIsoetes hyemalis sp. nov., is described from deciduous and mixed forest swamps. Populations are known from Virginia, South Carolina, and North Carolina with the greatest number occurring in the latter. Ecological, morphological, and geographic characteristics are described and discussed. -from Authors1 aBrunton, D.F.1 aBritton, D.M.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0028557718&partnerID=40&md5=c85aca50f0892224e4c64bf446144f7202366nas a2200145   4500008004100000245016300041210006900204260001200273300001400285490000700299520176600306100001502072700001702087856011602104       2001                        eng d00aThe utility of nuclear ITS, a LEAFY homolog intron, and chloroplast atpB-rbcL spacer region data in phylogenetic analyses and species delimitation in Isoëtes0 autility of nuclear ITS a LEAFY homolog intron and chloroplast at  c2001///  a166 - 1770 v913 aDespite its ancient origins, its worldwide distribution, and adaptation to diverse habitats, Isoëtes has a highly conserved morphology. This feature has made it difficult to resolve species and species relationships using morphological characters. In this paper, we report the utility of nucleotide sequences from the nuclear internal transcribed spacer (ITS) regions, chloroplast atpB/rbcL intergenic spacer region, and second intron of a LEAFY (LFY) homolog for identifying species relationships, delimiting basic diploid species, and determining hybrid origins. Variation in the ITS regions and atpB/rbcL spacer is most useful at the family level in Isoëtes and the LFY second intron is appropriate at the species and population level. The tree resulting from an analysis of the combined nuclear ITS and chloroplast atpB/rbcL spacer contains three major well supported clades (bootstrap ≥ 99%): an Old-World/California clade (I. abyssinica, I. longissima, I. velata, I. nuttallii, and I. orcuttii), an Asian/Australian clade (I. taiwanensis, I. japonica, I. kirkii, and I. drummondii), and a poorly resolved clade consisting of nine North American species. To further resolve and delimit the North American species, a combination of the LEAFY second intron and ITS data was used. The resulting consensus tree has limited resolution, supporting the hypothesis that the North American species complex radiated rapidly. The combination of LFY and ITS data provided numerous characters, both substitutions and indels, that are useful in species delimitation and identification of cryptic species. ITS sequence data, through additive banding and sequence misalignment, is also useful in confirming interspecific hybrids and determining their parental origins.1 aHoot, S.B.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0040332183&partnerID=40&md5=e97f5c223e9a884b67c6347a0db81dd501128nas a2200229   4500008004100000245010400041210006900145260001200214300001400226490000700240520039300247653001500640653001200655653001800667653001400685653001800699653001300717100002000730700001700750700001500767856011600782       2001                        eng d00aIsoetes mattaponica (Isoetaceae), a new diploid quillwort from freshwater tidal marshes of Virginia0 aIsoetes mattaponica Isoetaceae a new diploid quillwort from fres  c2001///  a200 - 2040 v113 aIsoetes mattaponica is a rare quillwort of freshwater tidal rivers of eastern Virginia with a somatic chromosome number of 2n = 22. Megaspores have low regulate muri on both the proximal and distal surfaces; the girdle is broad. Microspores are echinate-spinose with ornamented spines. Isoetes mattaponica may be one of the parents of several polyploids in the Southeastern United States.10aIsoetaceae10aIsoetes10aNorth America10aQuillwort10aUnited States10aVirginia1 aMusselman, L.J.1 aTaylor, W.C.1 aBray, R.D.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0034905622&partnerID=40&md5=0a918d8dca0158c2b1216f1b1f080cd001223nas a2200157   4500008004100000245007300041210006900114260001200183300001400195490000700209520068400216100001700900700001500917700001700932856011600949       2003                        eng d00aA re-evaluation of Isoetes savatieri franchet in Argentina and Chile0 areevaluation of Isoetes savatieri franchet in Argentina and Chil  c2003///  a126 - 1360 v933 aIsoetes suvutieri has traditionally been interpreted as being a uniform aquatic ranging from the southernmost regions of South America to the central Andes of Chile and Argentina. An examination of herbarium material supports the recognition of two taxa, a southern I. savatieri and a more northern I. chubutiana, from central Chile and Argentina. The latter taxon is hexaploid and described here as a new species. The morphology of these species suggest that they are sister species resulting from divergence following a polyploidy event. These species, and several other species pairs, provide the best and, to date, only examples of allopatric divergence in polyploid Isoetes.1 aHickey, R.J.1 aMacluf, C.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0141676348&partnerID=40&md5=323242638fd59ed4fe04b166c7422ad801188nas a2200241   4500008004100000245007900041210006900120260001200189300001400201490000700215520045700222653001000679653001400689653001500703653001200718653001400730653002700744100001600771700001200787700001700799700001400816856011600830       2002                        eng d00aIsoetes yunguiensis (Isoetaceae), a new basic diploid quillwort from China0 aIsoetes yunguiensis Isoetaceae a new basic diploid quillwort fro  c2002///  a587 - 5910 v123 aIsoetes yunguiensis, a basic diploid species with a chromosome number of 2n = 22, is described and illustrated. This species occurs on the Yunnan-Guizhou Plateau in southwest China. It is similar to I. japonica in its appearance and emergent aquatic habit. Herbarium specimens of I. yunguiensis previously have been misidentified as I. japonica. Isoetes yunguiensis differs from I. japonica in megaspore and microspore morphology and chromosome number.10aChina10aEast Asia10aIsoetaceae10aIsoetes10aQuillwort10aYunnan-Guizhou Plateau1 aWang, Q.-F.1 aLiu, X.1 aTaylor, W.C.1 aHe, Z.-R.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0036939584&partnerID=40&md5=da8d4d4668c03846b51b3df5877e860200726nas a2200133   4500008004100000245010000041210007000141260001200211300001400223490000700237520021500244100001700459856011600476       2002                        eng d00aIsoëtes x herb-wagneri, an interspecific hybrid of I. bolanderi x I. echinospora (Isoëtaceae)0 aIsoëtes x herbwagneri an interspecific hybrid of I bolanderi x I  c2002///  a161 - 1630 v923 aIsoëtes x herb-wagneri, the interspecific hybrid between Isoëtes bolanderi and I. echinospora, is described. The epithet commemorates Warren (Herb) Wagner for the direction and help he provided to the author.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0036555158&partnerID=40&md5=5756ed478e1a0ae4b05b7f08209f0c5d01867nas a2200181   4500008004100000245014500041210006900186260001200255300001400267490000700281520121700288100001101505700001301516700001101529700001101540700001701551856011701568       2004                        eng d00aChromosome behavior during meiosis and development of spore mother cells in the Chinese quillwort Isoetes sinensis T. C. Palmer (Isoetaceae)0 aChromosome behavior during meiosis and development of spore moth  c2004///  a183 - 1950 v943 aChromosome behavior during meiosis of the tetraploid Isoetes sinensis was observed to be similar to that of basic diploid Isoetes species. This observation was consistent with the previous report that polyploid species of Isoetes are allopolyploids. Meiosis was generally similar in both megaspore and microspore mother cells, but differences were observed. Due to the smaller size of the microspore mother cells, during prophase I, chromosomes are not as dispersed as those in megaspore mother cells. Cytomixis was observed in all stages of meiosis in microspore mother cells, but it was not seen at any stage of meiosis in megaspore mother cells. Cytomixis, lagging chromosomes, chromosome bridges, chromosome fragments, and micronuclei, observed during meiosis in I. sinensis, can affect sexual reproduction, but it does not appear to be a major cause of population decline in this species. The major cause of decline is habitat degradation from human disturbance. The few remaining populations of the Chinese quillwort are fragmented and the numbers of individuals contained in these populations dwindle as a result of habitat degradation. Conservation efforts are needed to save I. sinensis from extinction.1 aHe, Z.1 aWang, H.1 aLi, J.1 aYe, Q.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-11444270683&partnerID=40&md5=e3b19a6dd117ccd20573e526dceb63bf01961nas a2200193   4500008004100000245017900041210007100220260001200291300001400303490000700317520123100324100001701555700001901572700001501591700001201606700001701618700001501635856011701650       2004                        eng d00aPhylogenetic relationships of Isoëtes (Isoëtaceae) in China as revealed by nucleotide sequences of the nuclear ribosomal ITS region and the second intron of a LEAFY homolog0 aPhylogenetic relationships of Isoëtes Isoëtaceae in China as rev  c2004///  a196 - 2050 v943 aIsoëtes is an ancient lycopod lineage with a highly conserved morphology that provides few morphological characters to resolve the phylogeny of its species. Species appear to have evolved by divergence and allopolyploidy. The basic diploids I. hypsophila, I. taiwanensis, and I. yunguiensis and the tetraploid I. sinensis occur in China. Analysis of ITS sequences indicates that the Chinese Isoëtes species are part of an Australasian clade including I. brevicula from Western Australia and I. kirkii from New Zealand. Two distinct cloned sequences of the second intron of a LEAFY homolog were recovered from I. sinensis supporting the hypothesis that I. sinensis is an allotetraploid. One of the I. sinensis cloned sequences was similar to the I. taiwanensis sequence and the other cloned sequence was similar to the I. yunguiensis sequence identifying I. taiwanensis and I. yunguiensis as the likely parents of I. sinensis. Other cloned sequences recovered from I. sinensis were recombined parts of the two distinct sequences. Morphological evidence supporting an allotetraploid origin of I. sinensis is found in its larger microspore size and intermediate megaspore texture compared to I. taiwanensis, and I. yunguiensis.1 aTaylor, W.C.1 aLekschas, A.R.1 aQing, F.W.1 aLiu, X.1 aNapier, N.S.1 aHoot, S.B.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-11444262662&partnerID=40&md5=7b7a2b568e139bee1b60bd62836ff6ef02291nas a2200205   4500008004100000245010500041210007100146260001200217300001400229490000700243520160200250653001901852653001901871653001201890653001701902100001501919700001701934700001701951856011701968       2004                        eng d00aRevealing unknown or extinct lineages within Isoëtes (Isoëtaceae) using DNA sequences from hybrids0 aRevealing unknown or extinct lineages within Isoëtes Isoëtaceae   c2004///  a899 - 9040 v913 aIsoëtes, a heterosporous lycopod with a fossil record dating back to the Paleozoic, has numerous putative allopolyploids (resulting from hybridization events coupled with doubling of chromosome number). By using the highly variable nucleotide sequences from the second intron of a LFY homologue in Isoëtes, species could be delimited and hybrid origins determined. The data suggest that reticulate evolution is both common and complex within a more derived species complex of Isoëtes. Sequences of identifiable parentage and sequences that are unlike any diploid species known were recovered, leading to the conclusion that one or both of the putative parents have not yet been discovered or are extinct. A range of observations concerning allopolyploid speciation were categorized as follows: (1) verification of previous hypotheses regarding parentage (e.g., I. riparia, I. appalachiana), (2) determination that two morphologically distinct allotetraploid species can share the same parentage (I. azorica and I. acadiensis), (3) recognition of a cryptic allotetraploid species, indicated by the presence of different parental genomes (I. "appalachiana" from Florida), and (4) identification of allotetraploid species with one or two unknown parents (e.g., I. tuckermanii, I. acadiensis, I. azorica, and I. hyemalis). Some sequences from diploid species are remarkably uniform among populations (e.g., I. echinospora from various locations in North America, Iceland, and Wales), while others are variable at the subspecies level (e.g., northern and southern populations within I. engelmannii).10aAllopolyploidy10aHybrid origins10aIsoetes10aLEAFY intron1 aHoot, S.B.1 aNapier, N.S.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-11444256350&partnerID=40&md5=420a4ad06820ce82f60d7a70e7db9dec01566nas a2200169   4500008004100000245013100041210006900172260001200241300001400253490000700267520094200274100001501216700001601231700001601247700001701263856011601280       2003                        eng d00aRelationship between Water Chemistry and the Distribution of the Endangered Aquatic Quillwort Isoetes sinensis Palmer in China0 aRelationship between Water Chemistry and the Distribution of the  c2003///  a361 - 3670 v183 aThe progressive decline in populations of Isoetes sinensis Palmer in China has been attributed to deteriorating water quality; however, no data have been adduced to support this assumption. In this study we identified locations in China that presently have populations of I. sinensis and locations where the species has been extirpated. We measured various water chemistry parameters at these sites, as well as at similar sites within the distribution range of I. sinensis but from where the species has never been recorded. The results indicate that distribution and occurrence of I. sinensis are closely correlated with water chemistry. Water samples drawn from habitats from where I. sinensis has been extirpated had higher concentrations of heavy metal ions, particularly chromium VI (Cr 6+). In addition, these samples consistently yielded higher readings for dissolved carbon dioxide, water hardness, and concentration of nitrates.1 aWen, M.-Z.1 aPang, X.-A.1 aWang, Q.-F.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0242275988&partnerID=40&md5=c81ad2c1833c6a70e17163c958e43e5001167nas a2200217   4500008004100000245007400041210006900115260001200184300001400196490000700210520050300217653001000720653001400730653001100744653001500755653001400770100001300784700001800797700001700815856011700832       2005                        eng d00aIsoetes orientalis (Isoetaceae), a new hexaploid quillwort from China0 aIsoetes orientalis Isoetaceae a new hexaploid quillwort from Chi  c2005///  a164 - 1670 v153 aIsoetes orientalis is a previously undescribed, rare, and endangered quillwort of southeast China with a hexaploid chromosome count of 2n = 66. Megaspores of I. orientalis have a reticulate ornamentation consisting of irregularly anastomosing muri with ragged crests. Microspores have an echinate-tuberculate ornamentation. Herbarium specimens of I. orientalis previously have been misidentified as I. sinensis. Isoetes orientalis differs from I. sinensis in spore morphology and chromosome number.10aChina10aEast Asia10aHoetes10aIsoetaceae10aQuillwort1 aHong, L.1 aQing-Feng, W.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-18244377984&partnerID=40&md5=1809bda83eb743b4de93e257b51d634400495nas a2200157   4500008004100000245004200041210004200083260001200125300001200137490000700149100001700156700001700173700001300190700001700203856011700220       2005                        eng d00aConfirming dioecy in Isoëtes butleri0 aConfirming dioecy in Isoëtes butleri  c2005///  a85 - 870 v951 aTurner, N.A.1 aTaylor, W.C.1 aMasi, S.1 aStupen, M.E.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-22444443938&partnerID=40&md5=bc7628e7743a43de8d2db0b4c6ad2e7a01143nas a2200157   4500008004100000245013200041210006900173260001200242300001200254490000700266520055100273100001200824700001500836700001700851856011700868       2006                        eng d00aMorphological and anatomical variation in sporophylls of Isoetes sinensis palmer (Isoetaceae), an endangered quillwort in China0 aMorphological and anatomical variation in sporophylls of Isoetes  c2006///  a67 - 740 v963 aIsoetes sinensis is a rare and endangered plant in wetlands of southeastern China. Previous studies have reported the chromosome number, geographic distribution, and ecology of this Asian endemic, but there has not been an analysis of diagnostic characters associated with the sporophylls of I. sinensis in China. Therefore, morphological and anatomical variation of sporophylls in the three known Chinese populations of I. sinensis were evaluated and compared. The variation found is discussed in relation to the present taxonomy of I. sinensis.1 aLiu, H.1 aWang, Q.F.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-33845932936&partnerID=40&md5=dabaeae02209c3a5f4288b0f6d03a961