02081nas a2200241   4500008004100000245012600041210007000167260001200237300001200249490000700261520129100268653001701559653001501576653002201591653002301613653000901636100001601645700001201661700001601673700001701689700001601706856011701722       2005                        eng d00aGenetic variation within the endangered quillwort Isoëtes hypsophila (Isoetaceae) in China as evidenced by ISSR analysis0 aGenetic variation within the endangered quillwort Isoëtes hypsop  c2005///  a89 - 980 v823 aThe genetic diversity of 56 individuals of Isoëtes hypsophila Hand.-Mazz. from China was investigated by ISSR. Twelve primers were screened from 65 primers, and a total of 119 DNA fragments were scored, of these, 82% were polymorphic bands, which indicated that high levels of genetic variation existed in the natural populations. Genetic diversity varied greatly among populations with the percentage of polymorphic band (PPB) values ranging from 8 to 35%. An analysis of molecular variance (AMOVA) was used to apportion the variation between regions, among populations within regions, and within populations. Results indicated that most of the variance (85%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations, however, were only 5 and 10%, respectively. In the among-population analysis, the larger part of genetic variation (77%) resided among populations, and less (23%) presented differences within populations. UPGMA cluster analysis showed that there was no distinct genetic differentiation between populations from Sichuan province. A number of causes including limited gene flow, genetic drift and inbreeding might have led to these observed genetic profiles of I. hypsophila. © 2005 Elsevier B.V. All rights reserved.10aConservation10aEndangered10aGenetic variation10aIsoetes hypsophila10aISSR1 aChen, J.-M.1 aLiu, X.1 aWang, J.-Y.1 aRobert, G.W.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-20444420155&partnerID=40&md5=c58709593caeba345cf50c4fba7d621302062nas a2200229   4500008004100000245011900041210006900160260001200229300001400241490000800255520127100263653002101534653003101555653002301586653002601609653001901635100001601654700001201670700001701682700001601699856011701715       2008                        eng d00aChloroplast DNA phylogeography of the Chinese endemic alpine quillwort Isoetes hypsophila hand.-mazz. (isoetaceae)0 aChloroplast DNA phylogeography of the Chinese endemic alpine qui  c2008///  a792 - 7980 v1693 aWe examined chloroplast trnL intron sequence variation within Isoetes hypsophila, a Chinese endemic alpine quillwort species. Sequence data were obtained from 46 individuals in six populations throughout the natural distribution of the species. Sequences appeared to have neutral evolution (Tajima's criterion D = -1.66453, 0.1 &gt; P &gt; 0.05; Fu and Li's test D* = -1:92897, 0.1 &gt; P &gt; 0.05; F* = -2.17823, 0.1 &gt; P &gt; 0.05). Eleven haplotypes were identified in I. hypsophila. A relatively high level of haplotype diversity (h = 0.817) and a low level of nucleotide diversity (π = 0:0045) were detected in I. hypsophila. Indirect estimates of gene flow based on cpDNA variation revealed significant genetic differentiation between the ZD1 population in Yunnan Province and the populations from Sichuan Province. High genetic differentiation between Yunnan and Sichuan populations was consistently indicated by both hierarchical analyses of molecular variance (AMOVA; GST = 0.5011) and the structure of a neighbor-joining tree. From nested clade analysis, restricted gene flow with isolation by distance was inferred to be responsible for the current spatial genetic structure of I. hypsophila. © 2008 by The University of Chicago. All rights reserved.10aAlpine quillwort10acpDNA trnL intron sequence10aIsoetes hypsophila10aNested clade analysis10aPhylogeography1 aChen, J.-M.1 aLiu, F.1 aGituru, W.R.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-47649125533&partnerID=40&md5=8f27b208869f24aef198146456c288ea