02062nas a2200229   4500008004100000245011900041210006900160260001200229300001400241490000800255520127100263653002101534653003101555653002301586653002601609653001901635100001601654700001201670700001701682700001601699856011701715       2008                        eng d00aChloroplast DNA phylogeography of the Chinese endemic alpine quillwort Isoetes hypsophila hand.-mazz. (isoetaceae)0 aChloroplast DNA phylogeography of the Chinese endemic alpine qui  c2008///  a792 - 7980 v1693 aWe examined chloroplast trnL intron sequence variation within Isoetes hypsophila, a Chinese endemic alpine quillwort species. Sequence data were obtained from 46 individuals in six populations throughout the natural distribution of the species. Sequences appeared to have neutral evolution (Tajima's criterion D = -1.66453, 0.1 &gt; P &gt; 0.05; Fu and Li's test D* = -1:92897, 0.1 &gt; P &gt; 0.05; F* = -2.17823, 0.1 &gt; P &gt; 0.05). Eleven haplotypes were identified in I. hypsophila. A relatively high level of haplotype diversity (h = 0.817) and a low level of nucleotide diversity (π = 0:0045) were detected in I. hypsophila. Indirect estimates of gene flow based on cpDNA variation revealed significant genetic differentiation between the ZD1 population in Yunnan Province and the populations from Sichuan Province. High genetic differentiation between Yunnan and Sichuan populations was consistently indicated by both hierarchical analyses of molecular variance (AMOVA; GST = 0.5011) and the structure of a neighbor-joining tree. From nested clade analysis, restricted gene flow with isolation by distance was inferred to be responsible for the current spatial genetic structure of I. hypsophila. © 2008 by The University of Chicago. All rights reserved.10aAlpine quillwort10acpDNA trnL intron sequence10aIsoetes hypsophila10aNested clade analysis10aPhylogeography1 aChen, J.-M.1 aLiu, F.1 aGituru, W.R.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-47649125533&partnerID=40&md5=8f27b208869f24aef198146456c288ea01875nas a2200229   4500008004100000245009000041210006900131260001200200300001200212490000600224520112400230653001701354653002201371653001201393653004401405653002401449100001301473700001701486700001201503700001301515856011701528       2007                        eng d00aGenetic diversity in Isoetes yunguiensis, a rare and endangered endemic fern in China0 aGenetic diversity in Isoetes yunguiensis a rare and endangered e  c2007///  a46 - 490 v23 aIsoetes yunguiensis is an endangered and endemic fern in China. Field survey indicated that only one population and no more than 50 individuals occur in the wild. The genetic variation of 46 individuals from the population remaining at Pingba (Guizhou Province, China) was assessed by Random Amplified Polymorphic DNA (RAPD) finger-printing. Twelve primers were screened from sixty ten-bp arbitrary primers, and a total of 95 DNA fragments were scored. Of these, 62.1% were polymorphic loci, which indicated that high level genetic variation existed in the natural population. The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I. yunguiensis population. However, with the continuing decrease of population size, the genetic diversity will gradually be lost. We suggest that the materials from the extant population should be used for re-establishment of the populations. © 2007 Higher Education Press and Springer-Verlag.10aEndemic fern10aGenetic diversity10aIsoetes10aRandom amplified polymorphic DNA (RAPD)10aRare and endangered1 aChen, J.1 aGituru, W.R.1 aLiu, X.1 aWang, Q.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-33847396236&partnerID=40&md5=ee6f56a9a5fcf2d93d7218f43862d556