01250nas a2200217   4500008004100000245007400041210006900115260001200184300001400196490000700210520056500217653001200782653002600794653002200820653001600842653001600858100001600874700001300890700001200903856011700915       2008                        eng d00aTwo new species of Isoetes (Isoetaceae) from Jeju Island, South Korea0 aTwo new species of Isoetes Isoetaceae from Jeju Island South Kor  c2008///  a354 - 3580 v513 aWe name and describe two new species, Isoetes jejuensis and I. hallasanensis, from Jeju Island, South Korea. These taxa are compared with related species in East Asia, including I. coreana and I. sinensis. The rugulate megaspore ornamentation of I. jejuensis differs from those other Isoetes species. Furthermore, I. hallasanensis varies from I. asiatica in its microspore ornamentation and chromosome number, although both species have an echinate megaspore ornamentation. These new species are tetraploid (2n = 44), whereas I. coreana is hexaploid (2n = 66).10aIsoetes10aIsoetes hallasanensis10aIsoetes jejuensis10aJeju Island10aNew species1 aChoi, H.-K.1 aJung, J.1 aKim, C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-54249107417&partnerID=40&md5=b04955ae3773d8cafcac8c63a387913702208nas a2200217   4500008004100000245005800041210005600099260001200155300001400167490000700181520157700188653001001765653001401775653001201789653001101801653002101812100001201833700001201845700001601857856011701873       2008                        eng d00aSpore morphology of Isoëtes (isoëtaceae) from China0 aSpore morphology of Isoëtes isoëtaceae from China  c2008///  a479 - 4890 v463 aMicromorphological characters of mega- and microspores of five Isoëtes species from China were observed under scanning electron microscope (SEM). Megaspores of I. hypsophila have a levigate ornamentation, and are 358 μm in diameter (mean, n=30), microspores of this species have a gyrus ornamentation, and are 22 μm in length (mean, n=30); megaspores of I. yunguiensis have a cristate-reticulate ornamentation, with a size of 390 μm in diameter (mean, n=30), while microspores nearly smooth with some fine granulates, and with a size of 22 gm in length (mean, n=30); megaspores of I. taiwanensis are tuberculate and 312 μm in diameter (mean, n=30), echinate microspores are 24 μm in length (mean, n=30); cristate megaspores of I. sinensis are 409 μm in diameter (mean, n=30), and echinate microspores are 28 μm in length (mean, n=30); megaspores of I. orientalis have a cristate-reticulate ornamentation, with diameter of 420 μm (mean, n=30), microspores of this species have an echinate-tuberculate ornamentation with a size of 34 μm in length (mean, n=30). Based on the morphological characters of spores and chromosome numbers among taxa, it was found that mean spore size in each species of Isoëtes generally reflected the ploidy level. I. hypsophila, I. yunguiensis and I. taiwanensis, with smaller mean spore size, are diploidy with 2n=22; I. sinensis (2n=44) and I. orientalis (2n=66), with larger mean spore size, are polyploidy. The spore morphology of Isoëtes still plays an important role in identification and classification of Isoëtes from China.10aChina10aEvolution10aIsoetes10aOrigin10aSpore morphology1 aLiu, X.1 aLiu, H.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-57349117891&partnerID=40&md5=f310ff0165e440259efb4b7c6431474302236nas a2200229   4500008004100000245011500041210006900156260001200225300001200237490000700249520148500256653001701741653001401758653002201772653002001794653002501814653000901839100001201848700001301860700001601873856011701889       2008                        eng d00aGenetic diversity and population structure of endangered Isoetes coreana in South Korea based on RAPD analysis0 aGenetic diversity and population structure of endangered Isoetes  c2008///  a43 - 490 v893 aIsoetes coreana Chung and Choi is on the list of critically endangered species in South Korea. Using randomly amplified polymorphic DNA (RAPD) markers, we investigated the genetic diversity within and between seven local populations of I. coreana. Ten RAPD primers produced a total of 94 bands, of which 59 (62.8%) were polymorphic. A low level of genetic diversity was recognized within the populations of I. coreana: polymorphic loci (P), with values ranging from 3.4% to 33.9%, and a mean value of 15.5% being observed. Analysis of molecular variance (AMOVA) showed that genetic diversity was greater among populations (81.6%) than within populations (19.7%) or among the three regions included in this study (Han River, Youngsan River, and Nakdong River). In addition, a high degree of genetic differentiation (θB = 0.742) was detected among the populations. These results indicate that genetic differentiation has occurred very rapidly. However, the rate of gene flow between populations was found to be as low as 0.059, irrespective of the genetic and geographical distances between the populations, which indicates that genetic drift must have played an important role in forming the present populations of I. coreana. Because a reduction of genetic diversity as a result of genetic drift is undesirable, increasing the gene flow between populations of Korean quillwort I. coreana should be considered as a conservation strategy. © 2008 Elsevier B.V. All rights reserved.10aConservation10aGene flow10aGenetic diversity10aIsoetes coreana10aPopulation structure10aRAPD1 aKim, C.1 aNa, H.R.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-43049156535&partnerID=40&md5=6ec64294ed27758f6ffbdd47207484c002062nas a2200229   4500008004100000245011900041210006900160260001200229300001400241490000800255520127100263653002101534653003101555653002301586653002601609653001901635100001601654700001201670700001701682700001601699856011701715       2008                        eng d00aChloroplast DNA phylogeography of the Chinese endemic alpine quillwort Isoetes hypsophila hand.-mazz. (isoetaceae)0 aChloroplast DNA phylogeography of the Chinese endemic alpine qui  c2008///  a792 - 7980 v1693 aWe examined chloroplast trnL intron sequence variation within Isoetes hypsophila, a Chinese endemic alpine quillwort species. Sequence data were obtained from 46 individuals in six populations throughout the natural distribution of the species. Sequences appeared to have neutral evolution (Tajima's criterion D = -1.66453, 0.1 &gt; P &gt; 0.05; Fu and Li's test D* = -1:92897, 0.1 &gt; P &gt; 0.05; F* = -2.17823, 0.1 &gt; P &gt; 0.05). Eleven haplotypes were identified in I. hypsophila. A relatively high level of haplotype diversity (h = 0.817) and a low level of nucleotide diversity (π = 0:0045) were detected in I. hypsophila. Indirect estimates of gene flow based on cpDNA variation revealed significant genetic differentiation between the ZD1 population in Yunnan Province and the populations from Sichuan Province. High genetic differentiation between Yunnan and Sichuan populations was consistently indicated by both hierarchical analyses of molecular variance (AMOVA; GST = 0.5011) and the structure of a neighbor-joining tree. From nested clade analysis, restricted gene flow with isolation by distance was inferred to be responsible for the current spatial genetic structure of I. hypsophila. © 2008 by The University of Chicago. All rights reserved.10aAlpine quillwort10acpDNA trnL intron sequence10aIsoetes hypsophila10aNested clade analysis10aPhylogeography1 aChen, J.-M.1 aLiu, F.1 aGituru, W.R.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-47649125533&partnerID=40&md5=8f27b208869f24aef198146456c288ea