02236nas a2200229   4500008004100000245011500041210006900156260001200225300001200237490000700249520148500256653001701741653001401758653002201772653002001794653002501814653000901839100001201848700001301860700001601873856011701889       2008                        eng d00aGenetic diversity and population structure of endangered Isoetes coreana in South Korea based on RAPD analysis0 aGenetic diversity and population structure of endangered Isoetes  c2008///  a43 - 490 v893 aIsoetes coreana Chung and Choi is on the list of critically endangered species in South Korea. Using randomly amplified polymorphic DNA (RAPD) markers, we investigated the genetic diversity within and between seven local populations of I. coreana. Ten RAPD primers produced a total of 94 bands, of which 59 (62.8%) were polymorphic. A low level of genetic diversity was recognized within the populations of I. coreana: polymorphic loci (P), with values ranging from 3.4% to 33.9%, and a mean value of 15.5% being observed. Analysis of molecular variance (AMOVA) showed that genetic diversity was greater among populations (81.6%) than within populations (19.7%) or among the three regions included in this study (Han River, Youngsan River, and Nakdong River). In addition, a high degree of genetic differentiation (θB = 0.742) was detected among the populations. These results indicate that genetic differentiation has occurred very rapidly. However, the rate of gene flow between populations was found to be as low as 0.059, irrespective of the genetic and geographical distances between the populations, which indicates that genetic drift must have played an important role in forming the present populations of I. coreana. Because a reduction of genetic diversity as a result of genetic drift is undesirable, increasing the gene flow between populations of Korean quillwort I. coreana should be considered as a conservation strategy. © 2008 Elsevier B.V. All rights reserved.10aConservation10aGene flow10aGenetic diversity10aIsoetes coreana10aPopulation structure10aRAPD1 aKim, C.1 aNa, H.R.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-43049156535&partnerID=40&md5=6ec64294ed27758f6ffbdd47207484c002016nas a2200241   4500008004100000245012000041210007100161260001200232300001400244490000700258520124700265653000901512653002001521653001601541653001201557653002401569653001001593100001201603700001301615700001301628700001601641856011701657       2009                        eng d00aSystematic evaluation of isoëtes asiatica makino (isoëtaceae) based on AFLP, nrITS, and chloroplast DNA sequences0 aSystematic evaluation of isoëtes asiatica makino isoëtaceae base  c2009///  a501 - 5100 v523 aOn the basis of amplified fragment length polymorphism (AFLP) and nucleotide sequence data from nuclear ribosomal internal transcribed spacer (nrITS) and three chloroplast DNA regions (rbcL, cpITS, and trnS-psbC spacer), we investigated the species delimitation and the evolutionary lineage of Isoëtes asiatica from Hokkaido, Japan. The neighbor-joining (NJ) dendrogram based on AFLP markers revealed the well-defined clusters (bootstrap value = 100%) of I. asiatica. Results from the principal component analysis are largely congruent with those obtained in the NJ dendrogram. The maximum parsimony analysis, based on data from nrITS and three chloroplast DNA sequences, supported a monophyly of three species, I. asiatica, Isoëtes echinospora, and Isoëtes maritima from Hokkaido, Kamchatka, and Alaska regions. The distinct species status of I. asiatica was also well supported in the combined chloroplast DNA phylogeny. Therefore, I. asiatica appear to represent example of gradual speciation due to spatial isolation of ancestral populations followed by genetic divergence. Our results also suggest that I. asiatica is probably not the ancestral diploid of the polyploids occurring in East Asia. © The Botanical Society of Korea 2009.10aAFLP10aChloroplast DNA10aI. asiatica10aIsoetes10aMolecular phylogeny10aNrITS1 aKim, C.1 aNa, H.R.1 aShin, H.1 aChoi, H.-K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-71249137227&partnerID=40&md5=029dcd01eab0eacc2d8c97b3953cb1ba