01499nas a2200181   4500008004100000245007600041210006900117260001200186300001400198490000700212520087300219653002301092653002501115653001701140653002601157100001701183856011701200       2005                        eng d00aThe vegetation of temporary ponds with Isoetes in the Iberian Peninsula0 avegetation of temporary ponds with Isoetes in the Iberian Penins  c2005///  a219 - 2300 v353 aThis study examines the floristical composition and the distribution pattern of the ephemeral quillwort swards found in the Iberian Peninsula. A systematic review based initially on 120 phytosociological relevés and using numerical analyses with classification (Euclidean distance and UPGMA and MISSQ) and ordination (principal component analysis - PCA) was carried out. Four groups corresponding to the following associations were recognized: Junco pygmaei-Isoetetum velati, Peplido hispidulae-Isoetetum delilei, Junco capitati-Isoetetum histricis, Isoetetum durieui. The study supports previous assumptions that, on the local scale within the Iberian Peninsula, temporary-pond plant-communities show greater diversity in western territories thus following the same pattern that occurs on the regional scale of the Mediterranean basin. © 2005 Gebrüder Borntraeger.10aIsoetid vegetation10aNumerical ordination10aVernal pools10aWestern Mediterranean1 aMolina, J.A.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-31344444941&partnerID=40&md5=1d0cd9578246354cb891004f5102f08c01644nas a2200145   4500008004100000245016200041210006900203260001200272300001400284490000700298520104300305100001801348700001501366856011701381       2005                        eng d00aTaxonomic reassessment of North American granite outcrop Isoetes species with emphasis on vegetative morphology and I. Piedmontana (Pfeiffer) reed sensu lato0 aTaxonomic reassessment of North American granite outcrop Isoetes  c2005///  a204 - 2210 v703 aA taxonomic study of collections previously identified as Isoetes melanospora, I. piedmontana, and I. tegetiformans was undertaken emphasizing vegetative characters. The taxonomic position of I. piedmontana was also assessed using cytogeography and a cursory examination of the allozyme locus TPI-2. Isoetes melanospora, I. piedmontana, and I. tegetiformans can be sufficiently distinguished using characters derived from leaves and corms. Isoetes piedmontana occurs as diploid, triploid, and allotetraploid entities. Diploids in the western and eastern parts of the geographic range are genetically distinct. Allotetraploids in eastern Alabama are composed of different genomes than those in North Carolina. Isoetes piedmontana is currently interpreted as an assemblage of morphologically indistinguishable species and is undergoing further taxonomic investigation. The typification of I. piedmontana is clarified, and a key with descriptions of the granite outcrop Isoetes species of the southeastern United States Piedmont is provided.1 aHeafner, K.D.1 aBray, R.D.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-26844507369&partnerID=40&md5=bf4117785fa3f8ff3d4bb96bd79adbc301730nas a2200217   4500008004100000245009400041210006900135260001200204300001400216490000800230520101600238653001901254653002301273653001501296653001801311653001301329653002301342100001701365700001301382856011701395       2005                        eng d00aA morphometric study of three closely related taxa in the European Isoetes velata complex0 amorphometric study of three closely related taxa in the European  c2005///  a459 - 4640 v1483 aIsoetes velata A. Braun is an amphibious plant with Mediterranean and Ibero-Atlantic distribution, showing marked morphological variability. Within the same geographical area, closely related restricted-range taxa are also present, including I. velata ssp. asturicense (restricted to mountainous areas of the north-west Iberian Peninsula) and I. boryana (restricted to the southern French Atlantic coast). These related taxa have unclear taxonomy and are both threatened. We performed a morphometric study of specimens of the three taxa. Our results suggest that I. velata ssp. asturicense is better considered as a distinct species, I. asturicense Laínz (Laínz), distinguishable from the other two taxa by mean megaspore length (N ≥ 30) < 360 μm. In contrast, our results do not support consideration of I. boryana as a separate species, and we here propose that this taxon be considered as a variety, I. velata A. Braun var. boryana M.I. Romero & C. Real stat. nov. © 2005 The Linnean Society of London.10aEndemic plants10aHabitats directive10aIsoetaceae10aPteridophytes10ataxonomy10aThreatened species1 aRomero, M.I.1 aReal, C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-23444461737&partnerID=40&md5=55726fd221d3cb5a1595b8d812d4fa7501521nas a2200145   4500008004100000245008600041210006900127260001200196300001300208490000700221520099800228100001501226700001701241856011701258       2005                        eng d00aComparative morphology of the glossopodia of three North American Isoetes Ligules0 aComparative morphology of the glossopodia of three North America  c2005///  a94 - 1140 v953 aOne of the most distinctive features of the heterosporous lycopsids is the presence of a ligule. This structure, currently found only in Isoetes and Selaginella, is comprised of a basally embedded glossopodium and a free distal tongue. Previous studies on Indian species have demonstrated small variations in glossopodia suggesting the possibility that this structure could have taxanomic use. Serial cross, paradermal, and sagittal sections of glossopodia from three different North American species, representing three ploidy levels, were made. Three-dimensional digital rendering of the glossopodia provided comparative data for the three North America species and allowed comparisons with previously published descriptions. In general, the shape of the glossopodium is similar in all three North American species. There are several structural differences among them, such as the shape of the cornua, the length of the medimoles, and the angle of the glossopodium relative to the leaf axis.1 aShaw, S.W.1 aHickey, R.J.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-27844529330&partnerID=40&md5=9334fdaf0c080402ef9a0ccef97c921002081nas a2200241   4500008004100000245012600041210007000167260001200237300001200249490000700261520129100268653001701559653001501576653002201591653002301613653000901636100001601645700001201661700001601673700001701689700001601706856011701722       2005                        eng d00aGenetic variation within the endangered quillwort Isoëtes hypsophila (Isoetaceae) in China as evidenced by ISSR analysis0 aGenetic variation within the endangered quillwort Isoëtes hypsop  c2005///  a89 - 980 v823 aThe genetic diversity of 56 individuals of Isoëtes hypsophila Hand.-Mazz. from China was investigated by ISSR. Twelve primers were screened from 65 primers, and a total of 119 DNA fragments were scored, of these, 82% were polymorphic bands, which indicated that high levels of genetic variation existed in the natural populations. Genetic diversity varied greatly among populations with the percentage of polymorphic band (PPB) values ranging from 8 to 35%. An analysis of molecular variance (AMOVA) was used to apportion the variation between regions, among populations within regions, and within populations. Results indicated that most of the variance (85%) occurred between Yunnan and Sichuan. The variances among populations within regions and within populations, however, were only 5 and 10%, respectively. In the among-population analysis, the larger part of genetic variation (77%) resided among populations, and less (23%) presented differences within populations. UPGMA cluster analysis showed that there was no distinct genetic differentiation between populations from Sichuan province. A number of causes including limited gene flow, genetic drift and inbreeding might have led to these observed genetic profiles of I. hypsophila. © 2005 Elsevier B.V. All rights reserved.10aConservation10aEndangered10aGenetic variation10aIsoetes hypsophila10aISSR1 aChen, J.-M.1 aLiu, X.1 aWang, J.-Y.1 aRobert, G.W.1 aWang, Q.-F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-20444420155&partnerID=40&md5=c58709593caeba345cf50c4fba7d621301167nas a2200217   4500008004100000245007400041210006900115260001200184300001400196490000700210520050300217653001000720653001400730653001100744653001500755653001400770100001300784700001800797700001700815856011700832       2005                        eng d00aIsoetes orientalis (Isoetaceae), a new hexaploid quillwort from China0 aIsoetes orientalis Isoetaceae a new hexaploid quillwort from Chi  c2005///  a164 - 1670 v153 aIsoetes orientalis is a previously undescribed, rare, and endangered quillwort of southeast China with a hexaploid chromosome count of 2n = 66. Megaspores of I. orientalis have a reticulate ornamentation consisting of irregularly anastomosing muri with ragged crests. Microspores have an echinate-tuberculate ornamentation. Herbarium specimens of I. orientalis previously have been misidentified as I. sinensis. Isoetes orientalis differs from I. sinensis in spore morphology and chromosome number.10aChina10aEast Asia10aHoetes10aIsoetaceae10aQuillwort1 aHong, L.1 aQing-Feng, W.1 aTaylor, W.C.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-18244377984&partnerID=40&md5=1809bda83eb743b4de93e257b51d634400495nas a2200157   4500008004100000245004200041210004200083260001200125300001200137490000700149100001700156700001700173700001300190700001700203856011700220       2005                        eng d00aConfirming dioecy in Isoëtes butleri0 aConfirming dioecy in Isoëtes butleri  c2005///  a85 - 870 v951 aTurner, N.A.1 aTaylor, W.C.1 aMasi, S.1 aStupen, M.E.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-22444443938&partnerID=40&md5=bc7628e7743a43de8d2db0b4c6ad2e7a01453nas a2200217   4500008004100000245010000041210006900141260001200210300001400222490000700236520073900243653001500982653001200997653001201009653002901021653001701050100001601067700001701083700001801100856011701118       2005                        eng d00aAnalysis of morphological and anatomical characteristics of Isoetes using isoetes tennesseensis0 aAnalysis of morphological and anatomical characteristics of Isoe  c2005///  a167 - 1820 v573 aThe three known populations of Isoetes tennesseensis were examined to document and analyze their morphological and anatomical characters. Characters examined included velum coverage, lacunae, leaf form and size, sporangial wall cells, and ligule and labium morphology. Three types of morphological patterns were found: stable, variable, and dimorphic. The discovery of dimorphism between mega- and microsporophylls for velum coverage, velum length, sporangium size, and ligule shape is of particular interest. This study presents a model for future work designed to complement current species descriptions and it establishes a baseline for morphological comparisons with other Isoetes. © 2005, by The New York Botanical Garden Press.10aDimorphism10aIsoetes10aLycopod10aMorphological characters10aPteridophyte1 aBudke, J.M.1 aHickey, R.J.1 aHeafner, K.D.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-21744447722&partnerID=40&md5=10d5e4181461bf404f341ea4a32c474002599nas a2200253   4500008004100000245015300041210006900194260001200263300001400275490000800289520174100297653002202038653001702060653002302077653002102100653001702121653001802138653001902156653000802175653001302183100001402196700001802210856011702228       2005                        eng d00aA new species of Isoetes (Isoetaceae) from Turkey, with a study of microphyll intercellular pectic protuberances and their potential taxonomic value0 anew species of Isoetes Isoetaceae from Turkey with a study of mi  c2005///  a213 - 2280 v1473 aIsoetes anatolica sp. nov. is described from a population growing in seasonal ponds of a mountain near the southern coast of the Black Sea in Bolu, Turkey. It is a robust, amphibious quillwort, characterized by semiterete, carnose microphylls, semicircular foliar section, smooth cuticle, prominent cuticular pegs, stomata, several collenchymatous strands, abundant pectic filaments and connections in the cells of the translacunar diaphragms, incurved alae, well developed ligula, small carnose labium, no velum, well-formed bulliform megaspores and obscurely muriform microspores. Plants were investigated anatomically and a description with additional diagnostic characters is included. Morphological affinities with other species of the genus are discussed. Intercellular pectic protuberances (IPP) were studied in the cells of the translacunar diaphragms of the microphylls of several species of Isoetes including I. anatolica. The IPP were examined to determine if they could provide diagnostic characters. They were detected with TBO and analysed using light and scanning electron microscopy. Types of IPP and species bearing them were as follows: warts in I. adspersa, I. andina, I. boliviensis, I. duriei, I. engelmannii, I. lechleri, I. longissima, I. melanopoda, I. storkii, I. velata. ssp. velata, and I. velata ssp. asturicense; warts and filaments in I. brochonii, I. lacustris, and I. setacea, and connections in I. anatolica and I. malinverniana. IPP are lacking in I. boryana, I. echinospora, I. histrix and I. novogranadensis. Combination of type, density, and distribution of IPP promises to be a useful vegetative character in a genus in which diagnostic characters are scarce. © 2005 The Linnean Society of London.10aIsoetes anatolica10aLeaf anatomy10aPectic connections10aPectic filaments10aPectic warts10aPteridophytes10aSeasonal ponds10aSEM10ataxonomy1 aPrada, C.1 aRolleri, C.H.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-13844276026&partnerID=40&md5=fa39504c212fa1cc83a20ce72e22ce7b01460nas a2200265   4500008004100000245007800041210006900119260001200188300001400200490000700214520065700221653001600878653001200894653001800906653001000924653002700934653001400961653001100975653001600986100001701002700002101019700001701040700002001057856011701077       2005                        eng d00aTwo new species of the genus Isoetes L. (Isoetaceae-Lycopsida) from India0 aTwo new species of the genus Isoetes L IsoetaceaeLycopsida from   c2005///  a109 - 1160 v543 aTwo new, primitive quillwort species, I. udupiensis and I. divyadarshanii, are described from peninsular India. Cytologically both species are diploid and unique for India in having 22 chromosomes without fragments. Megaspores of both species are monomorphic, whereas in the previously described species, they are di-to polymorphic. Isoetes udupiensis is unique in having deltoid-shaped megaspores and dominant microspory. The similarity in morphology of both species to subgenus Euphyllum and to the remaining Indian species of subgenus Isoetes, suggests that they may be connecting links between primitive and derived forms within the genus in India.10aChromosomes10aDiploid10aI. udupiensis10aIndia10aIsoetes divyadarshanii10aPhylogeny10aSpores10aSystematics1 aShukla, P.K.1 aSrivastava, G.K.1 aShukla, S.K.1 aRajagopal, P.K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-15944372672&partnerID=40&md5=cad8ccfe64fc2bc9f7b174901a9b815303003nas a2200229   4500008004100000245011300041210006900154260001200223300001600235490000700251520223800258653000902496653002802505653002202533653002102555653002502576653001702601100001302618700001102631700001402642856011702656       2005                        eng d00aGenetic consequence of restricted habitat and population decline in endangered Isoetes sinensis (Isoetaceae)0 aGenetic consequence of restricted habitat and population decline  c2005///  a1265 - 12740 v963 a• Background and Aims: Isoetes sinensis (Isoeteaceae) is a critically endangered aquatic quillwort in eastern China. Rapid decline of extant population size and local population extinction have occurred in recent years and have raised great concerns among conservationists. • Methods: Amplified fragment length polymorphisms (AFLPs) were used to investigate the genetic variation and population structure of seven extant populations of the species. • Key Results: Eight primer combinations produced a total of 343 unambiguous bands of which 210 (61·2 %) were polymorphic. Isoetes sinensis exhibited a high level of intra-population genetic diversity (H E) = 0·118; hs = 0·147; I = 0·192; P = 35·2 %). The genetic variation within each of the populations was not positively correlated with their size, suggesting recent population decline, which is well in accordance with field data of demographic surveys. Moreover, a high degree of genetic differentiation (FST = 0·535; G ST = 0·608; θB = 0·607) was detected among populations and no correlation was found between geographical and genetic distance, suggesting that populations were in disequilibrium of migration-drift. Genetic drift played a more important role than gene flow in the current population genetic structure of I. sinensis because migration of I. sinensis is predominantly water-mediated and habitat range was highly influenced by environment changes. • Conclusions: Genetic information obtained in the present study provides useful baseline data for formulating conservation strategies. Conservation management, including both reinforcement for in situ populations and ex situ conservation programmes should be carefully designed to avoid the potential risk of outbreeding depression by admixture of individuals from different regions. However, translocation within the same regional population should be considered as a measure of genetic enhancement to rehabilitate local populations. An ex situ conservation strategy for conserving all extant populations to maximize genomic representation of the species is also recommended. © The Author 2005. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved.10aAFLP10aGenetic differentiation10aGenetic diversity10aIsoetes sinensis10aPopulation structure10aPteridophyte1 aKang, M.1 aYe, Q.1 aHuang, H.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-27944462292&partnerID=40&md5=26404edf2320e666820f93f6dd369ebf