00915nas a2200145   4500008004100000245006800041210006600109300001200175490000600187520040600193100001700599700001900616700001700635856011700652       2012                        eng d00aExpanded distribution of ISOTËS BUTLERI (ISOËTACEAE) in Texas0 aExpanded distribution of ISOTËS BUTLERI ISOËTACEAE in Texas  a753-7570 v63 aMore than 125 Isoētes butleri populations are reported from 11 additional counties in north central Texas. These collections bridge the 450 km disjunction between Oklahoma and central Texas records. SEM examination of megaspore ornamentation and size confirm the determination. Descriptions of sites, population sizes, associated plants, soils, and species characteristics are included and discussed.1 aTaylor, K.N.1 aO'Kennon, R.J.1 aRehman, T.F.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84870904944&partnerID=40&md5=a02ed72cc65a5d4dba982d6cac472a0502533nas a2200169   4500008004100000245010800041210006900149300001000218490000700228520193000235100001502165700001502180700002002195700001602215700001502231856011702246       2012                        eng d00aA cost-effective model for preliminary site evaluation for the reintroduction of a threatened quillwort0 acosteffective model for preliminary site evaluation for the rein  a66-730 v223 aThe choice of suitable sites for establishment of a new population of a species is the first step in a translocation programme. However, evaluation of a large number of sites can be demanding of time and money; practitioners need time- and cost-effective evaluation tools. A predictive model for the preliminary evaluation of potential reintroduction sites was developed using the endangered quillwort Isoëtes malinverniana as a case study. The reliability of three water habitat variables (pH, conductivity, and clarity) as predictors of the presence/absence of I. malinverniana was tested. Three sets of logistic models were produced on the basis of the mean values of pH and conductivity measured over 3, 4 and 6months to understand whether frequency of measurement affected the reliability of the models. Models of each set were ranked according to AIC and their reliability was then tested using data from the literature. The conductivity of water and pH were the most effective predictors of the suitability of sites for the reintroduction of I. malinverniana. In particular there was a negative relationship between the presence of I. malinverniana and these variables. Because of seasonal fluctuations in the variables considered, at least 4months monitoring are required to obtain reliable results. Despite this, the method is still advantageous in terms of costs, compared with repeated measurements of a wide range of chemical characteristics at a large number of sites. The main goal of this method is to limit expensive chemical analysis to a few sites chosen after the exclusion of unsuitable sites, through application of the model. Considering the similar ecophysiological features of quillworts from oligotrophic waters, the same model or conceptual framework could be applied to other quillworts and isoetid species growing in degraded areas and needing active conservation. © 2012 John Wiley & Sons, Ltd.1 aAbeli, T.a1 aBarni, E.b1 aSiniscalco, C.b1 aAmosso, C.a1 aRossi, G.a  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84857030721&partnerID=40&md5=633d0f32c1ac448751ee631478663b5001347nas a2200193   4500008004100000245013900041210006900180260001200249300001400261490000700275520061600282653002000898653003400918653002600952653001900978653002300997100001601020856011701036       2012                        eng d00aDirectional selection of bisexual plants in natural populations of Isoetes pantii (Pteridophyta: Isoetaceae) with chromosome evolution0 aDirectional selection of bisexual plants in natural populations   c2012///  a103 - 1150 v543 aPopulation biology studies during 1966-2010 have revealed that the proportion of bisexual plants has declined in natural populations of Isoetes pantii at Narsinghgarh (Central India), where it has been growing with the parental species I. coromandelina L. and I. sampathkumaranii Rao. Since many fundamental discoveries have been observed from within the microsporangia (male sporangia) over four decades the decline of production of male sporangia by this species has been briefly presented in the light of chromosomal as well as ecological factors. Directional selection appears to have favoured this decline.10abisexual plants10adifferential sexual selection10adirectional selection10aIsoetes pantii10anatural population1 aGoswami, H.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84859148941&partnerID=40&md5=ca2f20773a816d764eafa178c18cb50a02028nas a2200265   4500008004100000245015000041210006900191260001200260300001200272490000700284520111700291653004701408653002001455653002101475653002001496653002301516653002001539653000801559653001101567653002201578100001401600700001401614700001701628856011701645       2012                        eng d00aMicromorphological approach to the systematics of Mediterranean Isoëtes species (Isoëtaceae, Lycopodiophyta): Analysis of the megaspore surface0 aMicromorphological approach to the systematics of Mediterranean   c2012///  a35 - 430 v513 aMegaspore ornamentation is one of the few morphological characters used in the taxonomy of the genus Isoëtes. In the present work, we test the application of this character for distinguishing some Isoëtes species occurring in the Mediterranean: Isoëtes sicula, which according to some authors should be included as a variety in I. histrix, and the recently described I. todaroana, whose affinity with other taxa is currently unknown. Two additional species (I. duriei, I. velata) were also included in the analysis. The megaspores were studied using scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and chemical treatment. In all species, the megaspore surface is covered by a siliceous coating; removal of this coating reveals an underlying three-dimensional network of fused rodlets. A unique pattern of this network occurs in I. sicula, supporting its separation from I. histrix. Comparison between the patterns observed in the examined Mediterranean species shows that I. todaroana is most similar to I. histrix. © 2012 Copyright 2012 Collegium Palynologicum Scandinavicum.10aenergy-dispersive X-ray spectroscopy (EDS)10aIsoëtes duriei10aIsoëtes histrix10aIsoëtes sicula10aIsoëtes todaroana10aIsoëtes velata10aSEM10aSicily10asiliceous coating1 aTroia, A.1 aBazan, G.1 aSchicchi, R.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84859208191&partnerID=40&md5=8268047dea3226ff247ba747f4878e5d02378nas a2200169   4500008004100000245008300041210007000124260001200194300001400206490000700220520179500227100002502022700001602047700001502063700001302078856011702091       2012                        eng d00aEffects of temperature on the phenology of germination of Isoëtes echinospora0 aEffects of temperature on the phenology of germination of Isoëte  c2012///  a141 - 1530 v843 aIsoëtes echinospora, a submerged aquatic quillwort, is native in northern latitudes and a rare glacial relict in mountain lakes in temperate Central Europe. A relic population of this quillwort in the Plešné jezero lake has recovered recently from a 30-year period of failure to reproduce caused by acidification. Early ontogenetic stages of the quillwort are considered to be the most vulnerable to environmental changes. Therefore, the objective of this study was to investigate the phenology of germination of I. echinospora. In a two-year experiment, we examined the time course of germination of micro- and macrospores and establishment of sporelings under (i) natural in situ conditions in the Plešné jezero lake and (ii) at various temperatures (6-17 °C) in the laboratory. We developed a mathematical model that describes the temperature-specific temporal changes in the early ontogeny of I. echinospora. Our experiments clearly show that spores do not germinate at once but gradually over time if exposed to favourable temperatures. Generally, percentage germination tended to increase during the course of a season under most temperature regimes but was inhibited at the lowest temperature. With increasing temperature, microspores germinated earlier and more successfully than macrospores, as described by the model. Sporelings also developed faster at the higher temperature. However, the highest temperature used in the experiments (17 °C) desynchronized the phenology of germination in I. echinospora as it resulted in the two types of spore not being available for fertilization at the same time. Thus, climate change might affect interactions between temperature and the phenology of quillwort reproduction and threaten the survival of this species in Central Europe.1 atvrtlíková, M., Č1 aZnachor, P.1 aNedoma, J.1 aVrba, J.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84856654849&partnerID=40&md5=8703fe17900e64b794da47ec2c7950fe01941nas a2200253   4500008004100000245015600041210006900197260001200266300001200278490000700290520107700297653001901374653002201393653001201415653002201427653001901449653002501468100001601493700001601509700001701525700001401542700001401556856011701570       2012                        eng d00aMicrosatellite analysis reveals the genetic structure and gene flow of the aquatic quillwort Isoetes sinensis, a critically endangered species in China0 aMicrosatellite analysis reveals the genetic structure and gene f  c2012///  a52 - 570 v963 aIsoetes sinensis is a critically endangered aquatic quillwort, occurring in two fragmented sites of China (Xiuning county of Anhui; Jiande county of Zhejiang). During a five-year period (2004-2009), the areas and sizes of the two populations diminished dramatically due to intensive human activities. Genetic structure of the species was investigated using simple sequence repeat makers (SSRs). For seven nuclear microsatellites, high levels of genetic diversity were found within populations (H E=0.324-0.447). Strong genetic differentiation was detected between populations (G ST=0.376), while weak genetic differentiation was found within populations (G ST=0.026-0.080). Notably, in contrast to the source-sink model suggested by previous study (Chen et al., 2009), the migration pattern of I. sinensis along the Xin'an River is best explained by the linear symmetrical non-adjacent flow model (LSNF), which indicates that intensive human activities of recent years have greatly affected the gene exchange pattern among I. sinensis subpopulations. © 2011 Elsevier B.V.10aAllotetraploid10aGenetic diversity10aIsoetes10aMigration pattern10aRiparian plant10aStepping-stone model1 aChen, Y.-Y.1 aKong, D.-R.1 aHuang, C.-H.1 aXu, Y.-X.1 aLi, Z.-Z.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84855293635&partnerID=40&md5=824e647a2989ecda4486a69d98cdaac201672nas a2200241   4500008004100000245016700041210006900208260001200277300001600289490000700305520084100312653001301153653001201166653002101178653001201199653002101211100001701232700001501249700001701264700001501281700001701296856011701313       2012                        eng d00aLate pleistocene and holocene history of genus isoetes L. (Lycopodiophyta) in the Western Rhodope Mountains, Bulgaria. New palynological and palaeoecological data0 aLate pleistocene and holocene history of genus isoetes L Lycopod  c2012///  a1405 - 14100 v653 aThe genus Isoetes is represented in Bulgaria by one species I. lacustris L. (lake quillwort). It inhabits the littoral areas of some lakes in the Pirin Mountain range. Fossil records of Isoetes in Bulgaria are very scarce. A new palynological study was conducted on sediments collected from Kupena Mire in the Western Rhodope Mountains. The results indicated that lake quillwort was abundant 30 000 years ago and its growth was associated with the presence of shallow lake basins. The species disappeared from the composition of the local flora as the sedimentation regime changed from lacustrine to marsh after the warming of the climate at the onset of the Holocene. This study confirmed the existence of refugial areas during the last glaciation in the mountains of Southeastern Europe, where a number of glacial relics had survived.10aBulgaria10aIsoetes10aLate pleistocene10aRefugia10aWestern rhodopes1 aLazarova, M.1 aIvanov, D.1 aBozilova, E.1 aTonkov, S.1 aSnowball, I.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84868586431&partnerID=40&md5=4ea4602b4d474115a8b0a8a76884dfc801651nas a2200229   4500008004100000245008000041210006900121260001200190300001400202490000700216520094000223653001601163653001001179653001501189653001001204653001801214100001601232700001601248700001901264700002101283856011701304       2012                        eng d00aComparative studies of six populations of Isoetes panchganiensis from India0 aComparative studies of six populations of Isoetes panchganiensis  c2012///  a667 - 6760 v363 aSix populations of Isoetes panchganiensis G.K.Srivast., D.D.Pant & P.K.Shukla growing in different environmental conditions of the Western Ghats have been studied to determine the range of variation within the species. The present investigation does not show any variation in habitat (aquatic), rhizomorph (trilobed), velum (fenestra), and presence or absence of peripheral strands; however, there is a subtle variation in the length of plants, number of leaves, size of megasporangia, and size and ornamentation of the megaspores. The basic organisation of the megaspores of all the populations is almost same, but the finer details of the ornamentation of the megaspores exhibit subtle differences. They may be retate to reticulate. The muri may be complete or incomplete. Megaspores of the Kolahapur population are the largest in diameter, whereas the megaspores of the Khinger population are the smallest in diameter. © TÜBİTAK.10aEnvironment10aIndia10aPopulation10aSpore10aWestern Ghats1 aYadav, B.B.1 aSingh, S.K.1 aSrivastava, M.1 aSrivastava, G.K.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84868559813&partnerID=40&md5=72bc02d773835931349c2beb1b350ac500997nas a2200133   4500008004100000245006100041210005900102260001200161300001400173490000700187520053500194100001700729856011700746       2012                        eng d00aNew site of isoëtes echinospora (isoëtaceae) in Poland0 aNew site of isoëtes echinospora isoëtaceae in Poland  c2012///  a185 - 1880 v193 aIsoëtes echinospora Durieu is a very rare species in Poland. In 2009, it was found in Lake Okońskie in the eastern part of the Pomeranian Lakeland (NW Poland). This is the ninth locality of this species in Poland. It was found at a depth of 0.1-1.0 m. The water is acidic and poor in dissolved salts, including calcium salts, but quite rich in humic substances. The population of I. echinospora is relatively numerous. The individuals form aggregations or occur together with other isoetids (I. lacustris and Lobelia dortmanna).1 aRekowska, E.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84865174622&partnerID=40&md5=467e40749c3d822889f68937a6a8f18f01741nas a2200205   4500008004100000245010200041210006900143260001200212300001200224490000700236520105300243653001301296653001401309653002601323100001801349700001801367700001501385700001801400856011701418       2012                        eng d00aDevelopment of megaspores and microspores in Isoetes japonica A. Br. (Lycopodiophyta: Isoetaceae)0 aDevelopment of megaspores and microspores in Isoetes japonica A   c2012///  a84 - 960 v513 aMicrospore and megaspore development in Isoetes japonica was studied using light, scanning electron and transmission electron microscopy. Differences in cytokinesis following meiosis result in monolete microspores with bilateral symmetry, and trilete megaspores with radial symmetry. Wall development follows essentially the same sequence in both microspores and megaspores, but with the wall of the latter being very much thicker. A significant difference was observed in the tetrad stage, when microspores have an essentially rounded outline with the plasma membrane folded into a crest at the site of the future aperture. Megaspores in the tetrad stage are strongly invaginated over their entire surface, creating numerous crests, and this appears to have a role in determining the morphology of the perispore. The outline shape of the megaspore perispore having been established in the tetrad stage, most of its substance is added later and is derived from the degenerating tapetum. © 2012 Copyright 2012 Collegium Palynologicum Scandinavicum.10aexospore10aperispore10asporoderm development1 aBlackmore, S.1 aTakahashi, M.1 aUehara, K.1 aWortley, A.H.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84863571691&partnerID=40&md5=f7e81951aa6adcdbb44db83d38c686dd01725nas a2200241   4500008004100000245012600041210006900167260001200236300001600248490000700264520092400271653002401195653002201219653001501241653002301256653002101279653001901300100001101319700001201330700001301342700001101355856011701366       2012                        eng d00aMicrosatellite primers in the endangered quillwort Isoetes hypsophila (Isoetaceae) and cross-amplification in I. sinensis0 aMicrosatellite primers in the endangered quillwort Isoetes hypso  c2012///  ae184 - e1860 v993 aPremise of the study: The first microsatellite primers were developed for Isoetes hypsophila, an endangered quillwort species endemic to the Qinghai-Tibetan Plateau in China, to further describe its genetic variability and population structure. We also examined their cross-amplification in a congeneric species, I. sinensis. Methods and Results: Using the Fast Isolation by AFLP of Sequences COntaining Repeats (FIASCO) protocol, nine microsatellite loci were isolated and characterized in 32 samples from four natural populations of I. hypsophila. The primers amplified di-and hexanucleotide repeats with three to 11 alleles per locus. Seven of nine primers were cross-amplified in I. sinensis with two to seven alleles per locus. Conclusion: The microsatellite loci primers will be useful for studies of genetic diversity and gene flow in natural populations of Isoetes species. © 2012 Botanical Society of America.10aCross-amplification10aGenetic diversity10aIsoetaceae10aIsoetes hypsophila10aIsoetes sinensis10aMicrosatellite1 aLi, Z.1 aHan, Q.1 aChen, Y.1 aLi, W.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84860573611&partnerID=40&md5=4de1802063e8ced0a9e32ab46966d21501131nas a2200229   4500008004100000245006300041210006200104260001200166300001400178490000800192520043100200653001900631653001400650653001500664653001500679653001300694100001800707700001900725700002300744700001700767856011700784       2012                        eng d00aIsoetes mourabaptistae, a new species from Southern Brazil0 aIsoetes mourabaptistae a new species from Southern Brazil  c2012///  a174 - 1800 v1023 aIsoetes mourabaptistae, a new species from southern Brazil, is described, illustrated, and compared to the most similar species. This new species is apparently restricted to southern Brazil, and is characterized by cristate to irregularly reticulate megaspores and microechinate microspores. It is an aquatic plant, occurring among submersed rocks along rivers, at about 9001100 m in elevation. © 2012, American Fern Society.10aaquatic plants10adiversity10aIsoetaceae10alycophytes10ataxonomy1 aPereira, J.B.1 aWindisch, P.G.1 aLorscheitter, M.L.1 aLabiak, P.H.  uhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84868016532&partnerID=40&md5=d3bc8e1e27c5d582b331a37819fb1514