|Publication Type:||Journal Article|
|Year of Publication:||2000|
|Authors:||R. J. Duff, Schilling E. E.|
|Journal:||American Fern Journal|
|Pagination:||51 - 59|
Restriction site mapping was used to characterize the chloroplast genome of the lycophyte, Isoëtes melanopoda. The Isoëtes chloroplast genome is approximately 139-145 kb in size with an inverted repeat of 12-13 kb. The gene content and consensus gene order are similar to that of Marchantia. A distinctive feature of the Isoëtes genome is an increased size of the small single copy (SSC) region possibly due to the insertion of a piece of DNA (3-8 kb) of unknown composition. The inferred insertion, along with a slightly larger inverted repeat, are responsible for the apparent size difference in the total genome relative to Marchantia. Patterns of restriction fragments were also consistent with the presence of a small inversion (2-3 kb) in the large single copy (LSC) region.