|Publication Type:||Journal Article|
|Year of Publication:||2007|
|Authors:||J. Chen, Gituru, W. R., Liu, X., Wang, Q.|
|Journal:||Frontiers of Biology in China|
|Pagination:||46 - 49|
|Keywords:||Endemic fern, Genetic diversity, Isoetes, Random amplified polymorphic DNA (RAPD), Rare and endangered|
Isoetes yunguiensis is an endangered and endemic fern in China. Field survey indicated that only one population and no more than 50 individuals occur in the wild. The genetic variation of 46 individuals from the population remaining at Pingba (Guizhou Province, China) was assessed by Random Amplified Polymorphic DNA (RAPD) finger-printing. Twelve primers were screened from sixty ten-bp arbitrary primers, and a total of 95 DNA fragments were scored. Of these, 62.1% were polymorphic loci, which indicated that high level genetic variation existed in the natural population. The accumulation of genetic variation in the history of the taxon and the apparent minimal reduction effect on genetic diversity following destruction of habitat might be responsible for the high level genetic diversity presently remaining in the I. yunguiensis population. However, with the continuing decrease of population size, the genetic diversity will gradually be lost. We suggest that the materials from the extant population should be used for re-establishment of the populations. © 2007 Higher Education Press and Springer-Verlag.
Genetic diversity in Isoetes yunguiensis, a rare and endangered endemic fern in China